小麦<em>TaSAP2</em>-<em>6A</em>基因标记与多种环境下的农艺性状相关

doi:10.3969/j.issn.1674-7968.2021.03.005

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Abstract Stress associated proteins (SAPs) are the A20/AN1 zinc-finger proteins which widely involved in plant response to abiotic stress. The present study investigated the correlation between TaSAP2-6A gene marker and agronomic traits in a variety of environments to provide a basis for the exploration and utilization of excellent allelic variation in wheat (Triticum aestivum). In this study, TaSAP2-6A gene (GenBank No. JQ768347.1) was isolated from wheat, with the full-length genome sequence of 3 409 bp including the upstream sequence (2 714 bp), 3' untranslated region (167 bp) and coding region (528 bp). The coding region encoded 175 amino acids consisting of an A20 domain and an AN1 domain. Using a set of 'Chinese Spring' nullisomic-tetrasomic lines, TaSAP2-6A gene was located on chromosome 6A. It was consistent with the predictions made from URGI (Unité de Recherche Génomique Info) website. The polymorphism of TaSAP2-6A gene sequence was detected by 32 wheat accessions with high diversity, and no nucleotide variation was detected in the coding region, but one InDel (insert-deletion, T/-) site was identified at 1 527 bp site of the upstream sequence. A dCAPS (derived cleaved amplified polymorphic sequence) marker was developed based on the InDel, named as InDel-1527. The genotypes of a natural population consisted of 323 accessions were detected by the marker InDel-1527 of TaSAP2-6A. Association analysis revealed that this marker was significantly associated with plant height, grain number per spike and grain yield per plant in ten environments (year×site×treatment) with well-watered, drought-stressed, heat-stressed, and drought-heat-stressed treatments. The InDel (T) was considered as the superior allelic variation to decrease plant height, and increase grain number per spike and grain yield per plant. The results provide a gene resource and its functional marker for marker-assisted selection in wheat.

Key words： Wheat TaSAP2-6A Functional marker Association analysis

Received: 05 August 2020 Published: 01 March 2021

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S512.1

Corresponding Authors: *jingruilian@caas.cn

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	Articles by authors
	WANG Yi-Xue
	LI Long
	MAO Xin-Guo
	WANG Jing-Yi
	CHANG Xiao-Ping
	JING Rui-Lian

Cite this article:

WANG Yi-Xue,LI Long,MAO Xin-Guo, et al. Wheat (Triticum aestivum) TaSAP2-6A Gene Marker Is Associated with Agronomic Traits in Multiple Environments[J]. 农业生物技术学报, 2021, 29(3): 453-460.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.03.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I3/453

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