Detection of Genetic Loci of Spike Number per Plant Response to Nitrogen Stress in Wheat (Triticum aestivum)
ZHANG Na1, ZHANG Xi-Lan2, ZHAO Ming-Hui3, QIAO Wen-Chen3, FU Xiao-Yi4, HE Ming-Qi4, SUN Li-Jing5, LI Hui5, ZHAO Yue-Xing6, JI Jun2,*
1 Xuzhou Institute of Agricultural Sciences in Xuhuai Region of Jiangsu, Xuzhou 221131, China; 2 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; 3 Institute of Dry Farming Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Hengshui 053000, China; 4 Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang 050041, China; 5 Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China; 6 Agriculture and Rural Area of Yongnian District, Handan 057150, China
Abstract As an important factor that affects the spike number per unit area of wheat (Triticum aestivum), spike number per plant (SNPP) is sensitive to nitrogen level. In order to detect loci of SNPP response to nitrogen stress and to assist selecting and improving the nitrogen use efficiency or nitrogen stress sustain ability of wheat varieties, genome wide association study (GWAS) was conducted. The association panel consisted of 132 wheat varieties and their advanced lines derived from the north Yellow and Huai Valley Wheat Region of China. SNPP in 8 environments under 2 different nitrogen levels and tolerant index (TI) of SNPP to low nitrogen stress were investigated. Affymetrix Wheat 55K SNP was used to achieved the genetic data. Finally, 4 loci were identified to associated with SNPP in two or more environments accounting for 10.20%~17.5% of the phenotypic variation. And 3 loci associated with TI of SNPP were detected in 2 environments with the phenotypic variation ranging from 11.4% to 16.3%. Evaluation of the genetic effects and frequency of the alleles in the significantly associated loci suggested that it was important to strengthen the selection of the AX-108733649-2B, AX-108889870-4A, AX-111667100-5B and AX-111039914-7D loci for genetic improvement of wheat varieties with high nitrogen use efficiency.
ZHANG Na,ZHANG Xi-Lan,ZHAO Ming-Hui, et al. Detection of Genetic Loci of Spike Number per Plant Response to Nitrogen Stress in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2021, 29(3): 435-442.
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