福建正红菇保护林中壳斗科外生菌根真菌多样性研究

doi:10.3969/j.issn.1674-7968.2020.05.002

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Abstract To protect and rationally utilize Russula resources and investigate the regulation of protective picking on the structure of ectomycorrhizal fungi in Fagaceae symbiotic host of mushroom Russula could provide a theoretical basis to improve Russula yield increasing in Natural Russula forest. In present study, using morphology analysis and ribosomal DNA internal transcribed spacer (rDNA ITS) sequencing, the dominant species was identified as Russula griseocarnosa in Fujian, while in some part of eastern Fujian, the major species was identified as R. rosea. In addition, the combination of morphology and rDNA ITS sequencing were used to compare the differences in the composition and abundence of ectomycorrhizal communities in Fagaceae symbiotic tree caused by non-protective and protective picking (that is, the last stubble of the previous year (about late August) all picked and not picked) in a Russula nature reserve area in Jianou, Fujian. A total of 34 OTUs (operational taxonomic units) was identified in ectomycorrhizal fungi from root tips of Fagaceae. Among them, 4 species, 9 genus with unknown species, 3 family with unknown genus and 1 order with unknown family were identified. The core communities were Lactarius, Thelephoraceae, Russula and Cortinarius. The protective picking of Russula in the last crop increased the abundance of Russula and Thelephoraceae in the second year, while the abundance of Lactarius decreased. The present study clarified the types of mycorrhizal fungi and found different harvesting of Russula in the last stubble could affect the symbiosis rate of Russula with the Fagaceae and flora structure of Fagaceae roots in the next year, which could provide a reference for the artificial promotion of Russula production as well as restoration and transformation of the Russula protected forest ecosystem.

Key words： Russula Ectomycorrhizal fungi Fagaceae

Received: 19 September 2019

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S324

Corresponding Authors: *gdlufafu@163.com

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	Articles by authors
	YU Wen-Ying
	HU Hong-Li
	BAO Jian-Dong
	PENG Ming-Hui
	LU Guo-Dong

Cite this article:

YU Wen-Ying,HU Hong-Li,BAO Jian-Dong, et al. Diversity Study on Ectomycorrhizal Fungi from Fagaceae Roots in Russula Nature Reserve Area in Fujian[J]. 农业生物技术学报, 2020, 28(5): 771-783.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.05.002 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I5/771

[1] 陈羽, 梁俊峰, 周再知, 等. 2010. 红菇和正红菇菌种接种三个乡土树种的苗期效果[J]. 广东林业科技, 26(01): 22-28.
(Chen Y, Liang J F, Zhou Z Z, et al.2010. The effect of seedling inoculation of three local tree species inoculated by Russula lepida and R. vinosa[J]. Guangdong Forestry Science and Technology, 26(01): 22-28.)
[2] 陈宇航, 陈政明. 2007. 菌根多样性对红楮林细根生物量及红菇采收量的影响[J]. 中国生态农业学报, 15(05): 171-173.
(Chen Y H, Chen Z M.2007. Effects of mycorrhiza diversity on fine root biomass and harvest of red mushroom in red oak forest[J]. Chinese Journal of Eco-Agriculture, 15(05): 171-173.)
[3] 范俐. 2006. 福建省红菇的地理分布及其依存的植被类型[J]食用菌, 38(4): 4-6.
(Fan L.2006. Geographical distribution of Russula in Fujian province and its dependent vegetation type field[J]. Edible Fungi, 38(4): 4-6.)
[4] 耿荣, 耿增超, 黄建, 等. 2015. 秦岭辛家山林区云杉外生菌根真菌多样性[J]. 微生物学报, 55(07): 905-915.
(Geng R, Geng Z C, Huang J, et al.2015. Diversity of ectomycorrhizal fungi of spruce in Xinjiashan forest area of Qinling Mountains[J].Acta Microbiologica Sinica, 55(07): 905-915.)
[5] 耿荣, 耿增超, 黄建, 等. 2016. 秦岭辛家山林区锐齿栎外生菌根真菌多样性[J]. 菌物学报, 35(07): 833-847.
(Geng R, Geng Z C, Huang J, et al.2016. Diversity of ectomycorrhizal fungi of sharp-toothed eel in Xinjiashan forest area of Qinling mountains[J]. Mycosystema, 35(07): 833-847.)
[6] 贺斐. 2016. 典型栎林土壤真菌及外生菌根真菌对立地环境与Cd污染的响应[D]. 硕士学位论文, 济南大学, 导师: 王慧, pp. 21-26.
(He F.2016. The response of typical oak forest soil fungi and ectomycorrhizal fungi to environment factors and Cd pollution [D]. Thesis for M.S., Jinan University, Supervisor: Wang H, pp. 21-26.)
[7] 黄福常, 莫天砚, 刘斌. 1998. 环境因素对正红菇纯培养的影响及红菇菌剂制备研究[J]. 广西农业大学学报, 17(1): 40-45.
(Huang F C, Mo T Q, Liu B.1998. Study on the influence of environmental factors on the culture of Russula and the preparation of dosage form of Russula[J]. Journal of Guangxi Agricultural University, 17(1): 40-45.
[8] 黄年来, 吴经纶. 1984. 福建省已知食用菌初步名录[J]. 武夷科学, 4(00): 13-21.
(Huang N L, Wu J L.1984. Preliminary list of known edible fungi in Fujian province[J].Wuyi Science, 4(00): 13-21.)
[9] 鞠洪波. 2005. 樟子松枯梢病微生态控制研究[D]. 博士学位论文, 东北林业大学, 导师: 迟德富, pp. 25. (Ju H B. 2005. The Research of microecological control on shoot blight of Pinus sylvestris var. mongolica[D].Thesis for Ph.D., Northeast Forestry University, Supervisor: Chi D F, pp. 25.)
[10] 唐超, 陈应龙, 刘润进. 2011. 菌根食用菌研究进展[J]. 菌物学报, 30(03): 367-378.
(Tang C, Chen Y L, Liu R J.2011. Research progress on mycorrhizal edible fungi[J]. Acta Fungi, 30(03): 367-378.)
[11] 李国杰, 文华安. 2009. 中国红菇属分类研究进展[J]. 菌物学报, 28(2): 303-309.
(Li G J, Wen H A.2009. Advances in classification of Chinese genus[J]. Mycosystema, 28(2): 303-309.)
[12] 李惠珍, 黄德鑫, 许旭萍, 等. 1998. 正红菇的化学成分的研究[J]. 菌物系统, 17(01): 68-74.
(Li H Z, Huang D X, Xu X P, et al.1998. Study on the chemical constituents of Russula griseocarnosa[J]. Mycosystema, 17(01): 68-74.)
[13] 刘润进, 陈应龙. 2007.菌根学[M]. 北京: 科学出版社, pp. 44-75.
(Liu R J, Chen Y L.2007. Mycorrhizology[M]. Science Press, Beijing: Science Press, pp. 44-75.)
[14] 娄小华, 甘耀坤, 王黎明, 等. 2007. 红菇提取液对甲醋所致氧化损伤的保护作用[J]. 毒理学杂志. 3(21): 225-226.
(Lou X H, Gan Y K, Wang L M, et al.2007. Protective effect of Russula extract on oxidative damage induced by methyl vinegar[J]. Journal of Toxicology, 3(21): 225-226.)
[15] 马丽珍. 2016. 建瓯市天然林正红菇资源现状及保护对策[J]. 防护林科技, 16(01): 73-74, 89.(Ma L Z. 2016. The current status and protection strategies of natural forest Russula resources in JianOu city[J].Protection Forest Science and Technology, 16(01): 73-74, 89.)
[16] 邵文. 2008. 建瓯下坑红菇自然保护区红菇适生环境的调查[J]. 亚热带农业研究, 4(01): 40-43.
(Shao W.2008. Investigat on the suitable environment of Russula in the JianOu Russula nature reserve[J]. Subtropical Agriculture Research, 4(01): 40-43.)
[17] 宋福强, 刘远开, 杜春梅, 等. 2011. 小兴安岭红松外生菌根真菌资源及响应面法优化Suillus grevillei培养基[J]. 自然资源学报, 26(03): 440-449.
(Song F Q, Liu Y K, Du C M, et al.2011. Diversity of ectomycorrhizal macro-fungi at Xiao Hinggan mountain and medium optimization for Suillus grevillei using response surface methodology[J]. Journal of Natural Resources, 26(03): 440-449.)
[18] 宋彦君, 季志平, 吕平会, 等. 2016. 镇安板栗外生菌根真菌多样性研究[J]. 西北林学院学报, 31(04): 188-194.
(Song Y J, Ji Z P, Lu P H, et al.2016. Study on the diversity of ectomycorrhizal fungi in chestnut of Zhenan[J]. Journal of Northwest Forestry University, 31(04): 188-194.)
[19] 王惠, 代力民, 邵国凡, 等. 2003. 辽宁丹东地区柞树菌根真菌生态分布的研究[J]. 应用生态学报, 14(12): 2149-2152.
(Wang H, Dai L M, Shao G F, et al.2003. Study on the ecological distribution of eucalyptus fungi in Dandong area of Liaoning province[J]. Chinese Journal of Applied Ecology,14(12): 2149-2152.)
[20] 韦仕岩, 其天砚, 刘斌, 等. 1998. 广西浦北六万山椎林的红菇及其生态环境的调查研究[J]. 广西农业大学学报, 17(1): 25-32.
(Wei S Y, Qi T Y, Liu B, et al.1998. Study on the Russula of Liuwanshan vertebrate forest in Pubei, Guangxi and its ecological environment[J]. Journal of Guangxi Agricultural University, 17(1): 25-32.)
[21] 肖冬来, 陈宇航, 杨菁, 等. 2013. 福建正红菇遗传多样性分析[J]. 福建农业学报, 28(09): 902-905.
(Xiao D L, Chen Y H, Yang J, et al.2013. Analysis of genetic diversity of Russula griseaocarnosa in Fujian province[J]. Fujian Journal of Agricultural Sciences, 28(09): 902-905.)
[22] 谢雪丹, 刘培贵, 于富强. 2010. 云南松幼苗上红菇类菌根真菌的物种多样性及其菌根形态[J]. 云南植物研究, 32(03): 211-220.
(Xie X D, Liu P G, Yu F Q.2010. Russula mycorrhizal species diversity and mycorrhizal morphology on Yunnan Pinus ensis seedlings[J]. Yunnan Plant Research, 32(03): 211-220.)
[23] 于富强, 肖月芹, 刘培贵. 2007. 云南松(Pinus yunnanensis)林外生菌根真菌的时空分布[J]. 生态学报, 27(06): 2325-2333.
(Yu F Q, Xiao Y Q, Liu P G.2007. Spatiotanporal distribution of ectomycorrhizal fungi in Pinus yunnanensis forests[J]. Acta Ecologica Sinica, 27(06): 2325-2333.)
[24] 张振核, 郑维鹏, 伊可儿, 等. 1996. 葡酒红菇的生态学研究[J]. 生态学报, 16(2): 208-211.
(Zhang Z H, Zheng W P, Yi K R, et al.1996. Ecological study on Russularosa[J]. Journal of Ecology, 16(2): 208-211.)
[25] Agerer R.2006. Fungal relationships and structural identity of thei rectomycorrhizae[J]. Mycologica Progress, 5(2): 67-107.
[26] Francisca R, Teresa V, Carolina V, et al.2018. Ectomycorrhizal fungal diversity and community structure associatedwith cork oak in different landscapes[J]. Mycorrhiza, 28(4): 357-368.
[27] Gardes M, Bruns T D.1993. ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts[J]. Molecular Ecology, 2(2): 113-118.
[28] Gebhardt S, Neubert K, Wöllecke J, et al.2007. Ectomycorrhiza communities of red oak (Quercus rubra) of different age in the Lusatian lignite mining district, East Germany[J]. Mycorrhiza, 17(2): 279-290.
[29] Kranabetter J M, Friesen J, Gamiet S, et al.2009. Epigeous fruiting bodies of ectomycorrhizal fungi as indicators of soil fertility and associated nitrogen status of boreal forests[J]. Mycorrhiza, 19(8): 535-548.
[30] Morris M H, Perez M A, Smith M E, et al.2009. Influence of host species on ectomycorrhizal communities associated with two co-occurring oaks (Quercus spp.) in a tropical cloud forest[J]. FEMS Microbiology Ecology, 69(2): 274-287.
[31] Nieto M P, Carbone S S.2009. Characterization of juvenile maritime pine (Pinus pinaster Ait.) ectomycorrhizal fungal community using morphotyping, direct sequencing and fruitbodies sampling[J]. Mycorrhiza, 19(2): 91-98.
[32] Palmer J M, Lindner D L, Volk T J.2008. Ectomycorrhizal charscterization of an American chestnut (Castanea dentata)-dominated community in Western Wisconsin[J]. Mycorrhiza, 19(1): 7-36.
[33] Shade A, Peter H, Allison S D, et al.2012. Fundamentals of microbial community resistance and resilience[J]. Fronties in Microbiology, 19(3): 417-417.
[34] Smith M E, Douhan G W, Rizzo D M.2007. Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots[J]. New Phytologist, 174(4): 847-863.
[35] Toju H, Yamamoto S, Sato H, et al.2013. Community composition of root-associated fungi in a Quercus-dominated temperate forest: 'Codominance' of mycorrhizal and root-endophytic fungi[J]. Ecology and Evolution, 3(5): 1281-1293.
[36] Twieg B D, Durall D M, Simard S W.2007. Ectomycorrhizal fungal succession in mixed temperate forests[J]. New Phytologist, 176(2): 437-447.
[37] Wang Q, He X H, Guo L D.2012. Ectomycorrhizal fungus communities of Quercus liaotungensis Koidz of differentages in a northern China temperate forest[J]. Mycorrhiza, 22(6): 461-470.
[38] Wang X H, Yang Z L, Li Y C, et al.2009. Russula griseaocarnosa sp. nov. (Russulaceae, Russulales), a commercially important edible mushroom in tropical China: Mycorrhiza, phylogenetic position, and taxonomy[J]. Nova Hedwigia, 88(2): 269-282.
[39] Wang Y, Sheng H F, He Y, et al.2012. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags[J]. Applied and Environental Microbiology, 78(23): 8264-8271.