马铃薯<em>StUBC12</em>基因的生物信息学及表达特性分析

doi:10.3969/j.issn.1674-7968.2020.05.003

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Abstract The ubiquitin-proteasome pathway is widely involved in various stages of plant growth and development. The ubiquitin-conjugating enzyme E2 is the key enzyme in the pathway of enzyme-catalyzed and plays an important role in process of substrate ubiquitination. In this study, the StUBC12 (GenBank No. XM_006343293.2) was cloned from the potato (Solanum tuberosun) cultivar 'Atlantic' leaf cDNA. The full-length of the gene was 2 112 bp, and the CDS region is 444 bp. It was a split gene containing 3 introns and 4 exons. Bioinformatics analysis of StUBC12 indicated that the gene was a non-transmembrane protein encoded by 148 amino acids with a typical UQ_con conserved domain. The qRT-PCR technology was used to analyze the gene expression level of roots, stems, leaves and flowers in potato cultivars 'Atlantic' and 'Qingshu 9'. The results showed that the gene expression level was the highest in leaves and flowers. The expression level in 'Atlantic' was significantly higher than 'Qingshu 9'. Under drought and salt stress, potato StUBC12 gene up-regulated to increase plant stress resistance. This study could provide a theoretical basis for further study of the potato stress-resistance mechanism and the functional of the StUBC12 gene.

Key words： Potato StUBC12 Bioinformatics analysis Expression analysis

Received: 16 October 2019

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Corresponding Authors: *hjsi@gsau.edu.cn

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	FU Xue
	TANG Xun
	LIU Wei-Gang
	LI Shi-Gui
	QI Xue-Hong
	ZHANG Ning
	SI Huai-Jun

Cite this article:

FU Xue,TANG Xun,LIU Wei-Gang, et al. Bioinformatics and Expression Characteristic Analysis of StUBC12 gene of Potato (Solanum tuberosum)[J]. 农业生物技术学报, 2020, 28(5): 784-793.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.05.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I5/784

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