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| Optimization of Genetic Transformation System of Anthurium andraeanum cv. Arizona and Petunia hybrida Chalcone Synthase Gene (PhCHS) Transferation |
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Abstract Anthurium andraeanum is one of the popular lunar new year flowers in recent years. In order to optimize genetic transformation system for Anthurium andraeanum, leaves of cultivar Arizona plantlets was used as explants and the recombinant plasmid pSN1301-HYG-PhCHS was transferred to A. andraeanum cv. Arizona mediated by Agrobacterium tumefaciens GV3101. The efficient transformation system for A. andraeanum cultivar Arizona was described as follows: Leaves were cut and pre-cultured for 3 d on the 1/3 MS culture medium, which contained 200 mg/L NH4NO3, 0.5 mg/L BA, 0.05 mg/L 2,4-D, 5 g/L Agar and 30 g/L Sucrose, and then infected with A. tumefaciens (OD600=0.5) for 10 min. The inoculated leaves were put on MS culture medium at 28 ℃ under dark conditions for 3 d, and then transferred to the callus-inducing medium contained 30 mg/L hygromycin for resistant screening. The percentage of resistant-calli was 5%~6%. In total 14 transgenic plants with hygromycin resistance were obtained in this study and detected by PCR, and 9 of them were verified to be positive transgenic plants. PCR analysis also confirmed that Petunia hybrida chalcone synthase gene (PhCHS) has been transferred into A. andraeanum cultivar Arizona. The results will provide the technical support for the improvement of transgenic method for Anthurium cultivars.
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Received: 10 September 2014
Published: 02 February 2015
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