Study on Construction of Expression Vector of BoTCTP in Cabbage (Brassica oleracea) and Transferring to Chinese Kale (Brassica alboglabra)
WANG Jing-Jing1,2,CAO Bi-Hao1,CHEN Guo-Ju1,CHEN Chang-Ming1,CHEN Qing-Hua1,LEI Jian-Jun1*
1 College of Horticulture, South China Agriculture University, Guangzhou 510642, China; 2 Dezhou Academy of Agricultural Sciences, Dezhou 253015, China
Abstract Chinese kale (Brassica alboglabra) is one of the important vegetables in the Cruciferous family. It is often subjected to various adverse effects during the cultivation process, causing great losses to the production. In order to improve the resistance of Chinese kale to stress, the specific primers were designed according to BoTCTP (translationally controlled tumor protein cloned from Brassica oleracea) in this research. The interest fragment was amplified by PCR, and then the overexpression vector was constructed and transferred into Chinese kale by Agrobacterium tumefaciens mediated. The transformants were confirmed by molecular methods and biological assay. Four transgenic plants were obtained, and then taken to design the experiments associated with growth and different stress, such as high temperature, low temperature, salt, heavy metal and so on. The results showed that the transgenic plants grew faster, stronger and had bigger leaves than non-transgenic plants. In the different stress condition, the transgenic plants had obviously stronger resistance than the control, which showed that the BoTCTP was closely related to the growth and had a regulatory effect on the stress resistance of Chinese kale. These results provide some scientific basis for the future research on the stress resistance of Chinese kale.
WANG Jing-Jing,CAO Bi-Hao,CHEN Guo-Ju, et al. Study on Construction of Expression Vector of BoTCTP in Cabbage (Brassica oleracea) and Transferring to Chinese Kale (Brassica alboglabra)[J]. 农业生物技术学报, 2019, 27(1): 23-33.
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