Abstract Four cultivars of pepper（Capsicum annuum L.）were successfully transformed with the CBF-4 gene by Agrobacterium mediated transformation. This study established a highly efficient regeneration system of pepper . The main factors influencing regeneration and transformation rate were discussed and the best conduction of regeneration and transformation were determined. The positive regeneraion was obtained.60 Km-resistant regenerated plants were obtained, 46 of which were positive by the analysis of PCR and RT-PCR.The rate of transgene is 76.67%.The morphological indexes of transgenetic plants showed that it was compact plant type with thicker, darker green and larger area leaves compared with the non-transgenetic plants. The enzyme activities of SOD, POD and MDA in transgenetic plants are higher than that of the non-transgenetic plant (control group) after the cold resistant treatment.
