济宁百日鸡pri-miR-458b基因多态性及其与产蛋性状的关联分析

doi:10.3969/j.issn.1674-7968.2022.04.009

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Abstract miR-458b-5p mediates the Wnt/β-catenin signaling pathway to regulates the development of chicken (Gallus gallus) ovarian follicles, thus affecting the level of laying in poultry. The SNP of pri-miR-458b affects the processing of miR-458b. To explore the association of pri-miR-458b gene polymorphisms with laying trait, in this study, SNPs in pri-miR-458b gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Jining Bairi chickens. The results showed that a total of 30 SNPs were detected in the pri-miR-458b gene, and 10 loci of them were related to laying traits. Among them, rs316393825, rs734005133, rs737338503, rs733344248, rs740622221 and rs733777709 were linkage imbalance. Their combined genotype significantly affected egg number from 42 to 49 week of age and egg number until 49 week of age. The dominant haplotype of AGCGCG could increase egg number from 42 to 49 week of age by 3.29 and egg number until 49 week of age by 8.08. Compared with the free energy of AGCGCG type, the free energy of GGCGCG type decreased by 3.3 kcal/mol, while that of GTTAAA type increased by 5.3 kcal/mol. The secondary structure stability of GGCGCG type was the highest, AGCGCG type was the second, and GTTAAA type was the lowest. This study provides valuable molecular genetic markers for auxiliary selection of laying traits in Jining Bairi chickens.

Key words： Jining Bairi chicken pri-miR-458b SNP Laying traits Association analysis

Received: 02 June 2021

ZTFLH:

S831.2

Corresponding Authors: *wangwenwen@sdau.edu.cn; tanghui@sdau.edu.cn

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	HAN Xu
	SONG Zhi-Fang
	TENG Jun
	SHEN Guo-Rui
	WU Wen-Hui
	WANG Wen-Wen
	TANG Hui

Cite this article:

HAN Xu,SONG Zhi-Fang,TENG Jun, et al. Polymorphisms of pri-miR-458b Gene and Its Association Analysis with Laying Performance in Jining Bairi Chickens (Gallus gallus)[J]. 农业生物技术学报, 2022, 30(4): 704-715.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.04.009 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I4/704

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