Abstract miR-458b-5p mediates the Wnt/β-catenin signaling pathway to regulates the development of chicken (Gallus gallus) ovarian follicles, thus affecting the level of laying in poultry. The SNP of pri-miR-458b affects the processing of miR-458b. To explore the association of pri-miR-458b gene polymorphisms with laying trait, in this study, SNPs in pri-miR-458b gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Jining Bairi chickens. The results showed that a total of 30 SNPs were detected in the pri-miR-458b gene, and 10 loci of them were related to laying traits. Among them, rs316393825, rs734005133, rs737338503, rs733344248, rs740622221 and rs733777709 were linkage imbalance. Their combined genotype significantly affected egg number from 42 to 49 week of age and egg number until 49 week of age. The dominant haplotype of AGCGCG could increase egg number from 42 to 49 week of age by 3.29 and egg number until 49 week of age by 8.08. Compared with the free energy of AGCGCG type, the free energy of GGCGCG type decreased by 3.3 kcal/mol, while that of GTTAAA type increased by 5.3 kcal/mol. The secondary structure stability of GGCGCG type was the highest, AGCGCG type was the second, and GTTAAA type was the lowest. This study provides valuable molecular genetic markers for auxiliary selection of laying traits in Jining Bairi chickens.
HAN Xu,SONG Zhi-Fang,TENG Jun, et al. Polymorphisms of pri-miR-458b Gene and Its Association Analysis with Laying Performance in Jining Bairi Chickens (Gallus gallus)[J]. 农业生物技术学报, 2022, 30(4): 704-715.
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