|
|
Polymorphism of MYF5 Gene and Its Association Analysis with Growth Traits in Sheep (Ovis aries) |
MENG Ke, ZHANG Tian-Wen, LIANG Peng, SHAO Shun-Cheng, ZOU Shi-Fan, RONG Xuan, QIANG Hao, FENG Deng-Zhen* |
School of Agriculture, Ningxia University, Yinchuan 750021, China |
|
|
Abstract The myogenic factor 5 (MYF5) gene is considered to be an important gene affecting muscle growth and development. The aim of this study was to investigate the effect of genetic variation of MYF5 gene on growth traits in sheep (Ovis aries), in order to provide effective molecular genetic markers for the breeding of new breeds (lines) of high quality meat sheep. Three SNP loci (124510044, 124509770 and 124507708) with polymorphism and significant interbreed differences were screened. The selected loci were examined by flight mass spectrometry in 383 sheep from the 3 crosses and correlated with growth traits at birth and 3 months of age. The results showed that locus 12451044 detected 3 genotypes, which were moderately polymorphic (0.25 ≤PIC< 0.50) in all populations; locus 124509770 detected 3 genotypes, which were low polymorphic (PIC< 0.25) in the H1 generation population and moderately polymorphic (0.25 ≤PIC< 0.50) in the other populations. Only one genotype deletion was detected at locus 124507708. In the H2 generation population, individuals with CT genotype at locus 124510044 had significantly higher body height at 3 months of age than TT genotype (P<0.05), and individuals with CG genotype at locus 124509770 had significantly higher body height at 3 months of age than GG genotype (P<0.05); individuals with GG genotype had significantly higher chest circumference at 3 months of age than CC genotype, regardless of generation (P<0.05 ). 124510044 and 124509770 loci were complete linkage disequilibrium, producing 4 haplotypes and 5 diploids; individuals with diploid H4H4 had significantly higher chest circumference at birth and at 3 months of age than H1H1 (P<0.05). The results of this study showed that genetic variation in the MYF5 gene has an effect on growth traits in sheep, and the loci 124510044 and 124509770 can be used as potential candidate genetic markers for growth and development and meat production performance in meat sheep.This study provides an important reference for the MYF5 gene in the selection and breeding of meat sheep, and provides a theoretical basis for molecular marker-assisted breeding in meat sheep.
|
Received: 21 May 2021
|
|
Corresponding Authors:
*fengdengzhen126@163.com
|
|
|
|
[1] 李昊, 葛翠翠, 冯帆, 等. 2019. 脂肪酸在育肥滩羊、小尾寒羊及滩寒杂交羊肌肉组织中的含量特征[J]. 食品研究与开发, 40(3): 165-169. (Li H, Ge CC, Feng F, et al.2019. Content characteristics of fatty acids in muscle tissue of fattening Tan sheep, small tail Han sheep and Tan Han hybrid sheep[J]. Food research and Development, 40(3): 165-169.) [2] 马晓萌, 轩俊丽, 王慧华, 等. 2016. 乌珠穆沁绵羊RIPK2基因多态性与生长性状的关联[J]. 中国农业科学, 49(07): 1391-1407. (Ma X M, Xuan J L, Wang H H, et al.2016. Association between RIPK2 gene polymorphism and growth traits in Ujimqin sheep[J]. Chinese Agricultural Sciences, 49(07): 1391-1407.) [3] 彭雅鑫, 刘军, 赵诗瑜, 等. 2021. 猪RXRB基因SNPs检测及其与生长育肥和繁殖性状的关联分析[J]. 畜牧兽医学报, 52(03): 596-609. (Peng YX, Liu J, Zhao SY, et al.2021. Detection of SNPs of RXRB gene and its association with growth, fattening and reproductive traits in pigs[J]. Acta Zoologica Veterinary Sinica, 52(03): 596-609.) [4] 乔国艳. 2020. 高山美利奴羊重要经济性状遗传参数估计和遗传评定[D]. 硕士学位论文, 中国农业科学院, 导师: 杨博辉. pp. 16-18. (Qiao G Y.2020. Estimation and genetic evaluation of important economic traits in Alpine Merino sheep [D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Suppervisor: Yang B H. pp. 16-18.) [5] 唐莹, 王金玉, 张跟喜, 等. 2014. MYF5基因8个SNPs位点与京海黄鸡生长和繁殖性状的关联分析[J]. 畜牧兽医学报, 45(06): 863-870. (Tang Y, Wang J Y, Zhang G X, et al.2014. Association analysis of eight SNPs of MYF5 gene with growth and reproductive traits of Jinghai yellow chicken[J]. Acta Zoologica Veterinary Sinica, 45(06): 863-870.) [6] 韦宏伟, 徐刚毅, 汪代华, 等. 2011. MYF5基因多态性与山羊生长性状相关分析[J]. 中国畜牧杂志. 47(07): 15-17. (Wei H W, Xu G Y, Wang D H, et al.2011. Correlation analysis of MYF5 gene polymorphism and Goat Growth Traits[J]. Chinese Journal of Animal Husbandry. 47(07): 15-17.) [7] 徐云碧, 杨泉女, 郑洪建, 等. 2020. 靶向测序基因型检测(GBTS)技术及其应用[J]. 中国农业科学, 53(15): 2983-3004. (Xu Y B, Yang Q N, Zheng H J, et al.2020. Targeted sequencing genotyping technique and its application[J]. Chinese Agricultural Sciences, 53(15): 2983-3004.) [8] Azimu W, Manatbay B, Li Y, et al.2018. Genetic diversity and population structure analysis of eight local chicken breeds of Southern Xinjiang[J]. British Poultry Science, 59(6): 629-635. [9] Cai S F, Zhu Q, Guo C L, et al.2020. MLL1 promotes myogenesis by epigenetically regulating MYF5[J]. Cell Proliferation, 53(2): e12744. [10] Chen X Y, Wang D, Xiang H, et al.2017. Mitochondrial DNA T7719G in tRNA-Lys gene affects litter size in Small-tailed Han sheep[J]. Journal of Animal Science and Biotechnology, 8(03): 568-573. [11] Conerly M L, Yao Z Z, Zhong J W, et al.2016. Distinct activities of MYF5 and MyoD indicate separate roles in skeletal muscle lineage specification and differentiation[J]. Developmental Cell, 36(4): 375-385. [12] Honer B D, Camp NJ.2004. Principal component analysis for selection of optimal SNP-sets that capture intragenic genetic variation[J]. Genetic Epidemiology, 26(1): 11-21. [13] Kumar R, Singh SP, Mitra A.2018. Short-hairpin mediated myostatin knockdown resulted in altered expression of myogenic regulatory factors with enhanced myoblast proliferation in fetal myoblast cells of goats[J]. Animal Biotechnology, 29(1): 59-67. [14] Ryu Y C, Lee E A, Chai H H, et al.2018. Effects of a novel p.A41P mutation in the swine myogenic factor 5 (MYF5) gene on protein stabilizing, muscle fiber characteristics and meat quality[J]. Korean Journal for Food Science of Animal Resources. 38(4): 711-717. [15] Santos C P, Aguiar A F, Giometti IC, et al.2018. High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats[J]. Lasers in Medical Science, 33(4): 843-850. [16] Singh S S, Neelesh S, Mrinmoy G, et al.2021. Differential expression patterns of myogenic regulatory factors in the postnatal longissimus dorsi muscle of Jeju native pig and berkshire breeds along with their co-expression with pax7-sciencedirect[J]. Electronic Journal of Biotechnology, 51: 8-16. [17] Villatoro K M, Yang F, Duarte T, et al.2021. Quality, proximate composition, and sensory characteristics of Dorper, domestic commercial crossbred, and Australian sheep meat: a comparative study[J]. Translational Animal Science, 5(1): txab024-txab024. [18] Wang J, Hu Y S, Elzo M A, et al.2017a. Genetic effect of MYF5 gene in rabbit meat quality traits[J]. Journal of Genetics, 96(4): 673-679. [19] Wang J Q, Zhou H T, Forrest R H J, et al.2017b. Variation in the ovine MYF5 gene and its effect on carcass lean meat yield in new zealand romney sheep[J]. Meat Science, 131: 146-151. [20] Wei Y, Zhang GX, Zhang T, et al.2016. MYF5 and MyoG gene SNPs associated with Bian chicken growth trait[J]. Genetics and Molecular Researc, 15(3): 1-9. [21] Wu J B, Cao L P, Li M M, et al.2013. MYF5 gene polymorphisms and production performance traits in songliao white geese[J]. Genetics and Molecular Research, 12(4): 6052-6058. [22] Yin Z Z, Dong X Y, Dong D J, et al.2016. Association of MYF5 and KLF15 gene polymorphisms with carcass traits in domestic pigeons (Columba livia)[J]. British Poultry Science. 57(5): 612-618. [23] Zammit P S.2017. Function of the myogenic regulatory factors MYF5, MyoD, Myogenin and MRF4 in skeletal muscle, satellite cells and regenerative myogenesis[J]. Seminars in Cell and Developmental Biology, 72: 19-32. [24] Zhao C P, Raza A H S, Khan R, et al.2020a. Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle[J]. Genomics, 112(4): 2804-2812. [25] Zhao M M, Tazumi A, Takayama S, et al.2020b. Induced fetal human muscle stem cells with high therapeutic potential in a mouse muscular dystrophy model[J]. Stem Cell Reports, 15(1): 80-94. |
|
|
|