纳米孔测序技术在植物病原检测中的应用与展望

doi:10.3969/j.issn.1674-7968.2021.09.016

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Abstract Plant pathogenic agents seriously threaten the yield and quality of crop. Establishing efficient and accurate pathogen diagnosis technology is critical for disease control. Metagenomic sequencing technology based on next generation sequencing (NGS) platform is an important tool for plant disease identification. However, the application of NGS in the rapid detection of plant pathogens is restricted by the disadvantages of high cost, long experimental period and bulky equipment. Nanopore sequencing technology developed by Oxford Nanopore Technology Co., Ltd. (ONT)(Oxford, United Kingdom) is a new sequencing platform. Compared with the NGS platform, it has advantages such as low cost, short experimental period and easy to use. It is suitable for field detection and has been used in pathogen detection. In this paper, the principle of ONT Nanopore sequencing technology was introduced, and the applications and challenges of this technology in plant pathogen detection were summarized, which provides reference for the further application and improvement of this technology in plant pathogen detection.

Key words： Nanopore sequencing Third generation sequencing technology Plant pathogen detection

Received: 03 November 2020

ZTFLH:

S41-30

Corresponding Authors: * sunkai0719@126.com, yuxiaoping19630306@163.com

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	Articles by authors
	LU Hui-Xin
	SUN Kai
	YIN Chuan-Lin
	HUANG Yu-Dong
	YU Xiao-Ping

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LU Hui-Xin,SUN Kai,YIN Chuan-Lin, et al. Application and Prospect of Nanopore Sequencing Technology in Plant Pathogen Detection[J]. 农业生物技术学报, 2021, 29(9): 1817-1824.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.09.016 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I9/1817

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