Establishment of Qualitative and Quantitative Detection Method for Transgenic Brassica napus NS-B50027-4
LEI Zhan1, WANG Jian-Cheng3, ZHANG Chen1, LI Kai2,3, HUANG Kun-Lun3, SHANG Ying1, XU Wen-Tao2,3
1 College of agriculture and food, Kunming University of Science and Technology, Kunming 650504, China;
2. Beijing Advanced Innovation Center for Food Nutrition and Human Health/Department of Nutrition and Health, China Agricultural University, Beijing 100083, China;
3 Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture / College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Abstract Genetically modified Brassica napus NS-B50027-4 is a new variety containing a high proportion of ω3 fatty acids and LC-ω3 fatty acids approved by the Australia-New Zealand Food Standards Agency. So far there is no literature report on the detection method of this new genetically modified B. napus variety. Therefore, it is necessary to establish a qualitative and quantitative detection method for this species. In this study, primers and probes were designed according to the internal reference gene HMG (high mobile group protein) of B. napus and A02 chromosome of transgenic B. napus NS-B50027-4, respectively. The specificity, sensitivity and accuracy of the primers and probes were determined by conventional PCR and qPCR technology to determine the detection limit and quantification limit of the detection method. The experimental results show that the two designed primers could only amplify the target, the detection limit of hmg primer can reach 5 copies/μL, and the detection limit of A02dn2 primer is 1 copy/μL. Therefore, the established qualitative and quantitative detection method of genetically modified B. napus NS-B50027-4 has high specificity and selectivity, excellent sensitivity and accuracy. The research results provide technical support for the qualitative and quantitative detection of genetically modified B. napus NS-B50027-4.
LEI Zhan,WANG Jian-Cheng,ZHANG Chen, et al. Establishment of Qualitative and Quantitative Detection Method for Transgenic Brassica napus NS-B50027-4[J]. 农业生物技术学报, 2021, 29(9): 1825-1835.
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