贝莱斯芽胞杆菌3A3-15菌株对盆栽大豆土壤细菌群落结构的影响

doi:10.3969/j.issn.1674-7968.2020.05.014

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Abstract Root rot is the main disease that leads to soybean (Glycine max) yield reduction. Preliminary work screened a strain named Bacillius velezensis 3A3-15 with strong antagonistic effect on Fusarium oxysporum which is pathogenic bacteria of root rot in the soybean. To investigate the effect of biocontrol B. velezensis 3A3-15 on bacterial community structure in rhizosphere soil of potted soybean, the pots containing F. oxysporum were divide into 2 groups, the experimental group used 3A3-15 agent in the pot soil and the control group did not use 3A3-15 agent. High throughput sequencing technique was used to determine the bacterial community structure of the rhizosphere soil. Taxonomy was used to annotate the OTU (operational taxonomic units) after grouping by using the usearch61 clustering method. Alpha diversity analysis showed that Shannon index, PD (phylogenetic diversity) value, Simpson index and Chao1 index in the test group decreased, but no significance. The test group had 2 258 OTUs (operational taxonomic units) specifically, decreased by 32.74% when compared with the control group (3357). Top 10 dominant microbial bacteria at phylum level were Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, Bacteroidetes, Chloroflexi, Verrucomicrobia, Planctomycetes, TM7 and Nitrospirae. There was no significant difference in the abundance of dominant phylum between the test and control group. However, the abundance of Archaea, Tracer, Fibrobacteria and OP11 in the test group was significantly higher than that in the control group (P<0.05), and the abundance of GN02 in the test group was significantly higher than that in the control group (P<0.01). The dominant species in the 2 groups were Kaistobacter and Sphingomonas. NMDS (nonmetric multidimensional scaling) analysis showed that the difference of bacterial community in test group was smaller than that in control group. In conclusion, the addition of Bacillus velezensis 3A3-15 increased the abundance of some non-dominant bacteria and reduced the differences of bacterial community structure, but these changes were no significant in the total abundance and diversity of soil bacteria between 2 groups. The above results could provide reference data for studying on biological control mechanism and safe application of 3A3-15 biocontrol agent.

Key words： Biocontrol bacteria Bacillus velezensis High throughput sequencing Soybean soil Bacterial community structure

Received: 11 October 2019

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Corresponding Authors: *gtgrxf@163.com

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	LIU Xue-Jiao
	YAO Yan-Hui
	LI Hong-Ya
	ZHANG Dong-Dong
	GAO Tong-Guo
	ZHU Bao-Cheng

Cite this article:

LIU Xue-Jiao,YAO Yan-Hui,LI Hong-Ya, et al. Effect of Bacillus velezensis 3A3-15 on Bacterial Community Structure of Potted Soybean (Giycine max) Soil[J]. 农业生物技术学报, 2020, 28(5): 903-910.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.05.014 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I5/903

[1] 郭荣君, 李世东, 张晶, 等. 2010. 基于营养竞争原理的大豆根腐病生防芽孢杆菌的筛选及其特性研究[J]. 植物病理学报, 40(3): 307-314.
(Guo R J, Li S D, Zhang J, et al.2010. Characterization of Bacillus straias screened via nutritionaI competition for biocontrol of soybean root rot disease[J]. Acta Phytopathologica Sinica, 40(3): 307-314.)
[2] 黄玲玲, 裘纪莹, 唐琳, 等. 2015. 解淀粉芽胞杆菌NCPSJ7对采后苹果轮纹病的生物防治作用[J]. 中国食物与营养, 21(2): 20-24.
(Huang L L, Qiu J Y, Tang L, et al.2015. Biocontrol effect of Bacillus amylolyticus NCPSJ7 on postharvest apple ring rot[J]. Food and Nutrition in Chinese, 21(2): 20-24.)
[3] 连彩, 郭晓军, 朱宝成, 等. 2012. 兰花枯萎病拮抗细菌的筛选与鉴定[J]. 华北农学报, 27(2): 222-225.
(Lian C, Guo X J, Zhu B C, et al.2012. Screening and identification of antagonistic bacteria against Colletoichum wilt[J]. Acta Agriculturae Boreali-Sinica, 27(2): 222-225.)
[4] 刘欣, 李志英, 刘瑞瑞, 等. 2018. 大豆不同生育期根际土壤细菌群落结构的变化[J]. 广西植物, 38(10): 1363-1370.
(Liu X, Li Z Y, Liu R R, et al.2018. Changes of bacterial flora structure in rhizosphere soil of soybean at different growth stages[J]. Guihaia, 38(10): 1363-1370.)
[5] 刘艳, 王振军. 2016. 黄瓜枯萎病生物防治作用机理初步研究以及研究进展[J]. 农业科技通讯, (10): 242-244.
(Liu Y, Wang Z J. 2016. Preliminary study on biological control mechanism of cucumber wilt and its research progress[J]. Bulletin of Agricultural Science and Technology, (10): 242-244.)
[6] 殷继忠, 李亮, 接伟光, 等. 2018. 连作对大豆根际土壤细菌菌群结构的影响[J]. 生物技术通报, 34(1): 230-238.
(Yin J Z, Li L, Jie W G, et al.2018. Effects of continuous cropping on bacterial flora structure in soybean rhizosphere soil[J]. Biotechnology Bulletin, 34(1): 230-238.)
[7] Amato K R, Yeoman C J, Kent A, et al.2013. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes[J]. The ISME Journal, 7(7): 1344-1353.
[8] Adesemoye A O, Obini M, Ugoji E O.2008. Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtllis in three vegetables[J]. Brazilian Journal of Microbiology, 39(3): 423-426.
[9] Bacon C W, Yates I E, Hinton D M.2001. Biological control of Fusarium moniliforme in maize[J]. Environmental Health Perspectives, 109(S2): 325-332.
[10] Caporaso J G, Kuczynski J, Stombaugh J, et al.2010. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 7(5): 335-336.
[11] Correa O S, Montechia M S, Berti M F.2009. Bacillus amyloliquefaciens BNM122, a potential microbial biocontrol agent applied on soybean seeds, causes a minor impact on rhizosphere and soil microbial communities[J]. Applied Soil Ecology, 41(2): 185-194.
[12] Desantis T Z, Hugenholtz P, Larsen N, et al.2006. Greengenes, achimera-checked 16S rRNA gene database and workbench compatible with ARB[J]. Applied and Environmental Microbiology, 72(7): 5069-5072.
[13] de Silva A, Patterson K, Rothrock C, et al.2000. Growth promotion of highbush blueberry by fungal and bacterial inoculants[J]. Horticultural Science, 35(7): 1228-1230.
[14] Dong L, Xu J, Zhang L, et al.2018. Rhizospheric microbial communities are driven by, Panax ginseng, at different growth stages and biocontrol bacteria alleviates replanting mortality[J]. Acta Pharmaceutica Sinica B, 8(2): 272-282.
[15] Edgar R C.2010. Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics, 26(19), 2460-2461.
[16] Khalid A, Arshad M, Zahir Z A.2004. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat[J]. Journal of Applied Microbiology, 96(3): 473-480.
[17] Larkin R P.2003. Characterization of soil microbial communities under different potato cropping systems by microbial population dynamics, substrate utilization, and fatty acid profiles[J]. Soil Biology and Biochemistry, 35(11): 1451-1466.
[18] Matos A, Kerkhof L, Garland J L.2005. Effects of microbial community diversity on the survival of Pseudomonas aeruginosa in the wheat rhizosphere[J]. Microbial Ecology, 49(2): 257-264.
[19] O'Mahony M M, Dobson A D, Barnes J D, et al.2006. The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil[J]. Chemosphere, 63(2): 307-314.
[20] Takahashi S, Tomita J, Nishioka K, et al.2014. Development of a prokaryotic universal primer for simultaneous analysis of bacteria and archaea using next-generation sequencing[J]. PLOS ONE, 21(9): e105592.
[21] Wessendorf J, Lingens F.1989. Effect of culture and soil conditions on survival of Pseudomonas fluorescens R1 in soil[J]. Applied Microbiology Biotechnology, 31(1): 97-102.
[22] Zheng H, Dietrich C, Radek R, et al.2016. Endomicrobium proavitum, the first isolate of Endomicrobia class. nov. (phylum Elusimicrobia)-an ultramicrobacterium with an unusual cell cycle that fixes nitrogen with a group Ⅳ nitrogenase[J]. Environmental Microbiology, 18(1): 191-204.