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| Identification of Hsp40 Family Gene and the Expression Analysis Under Heat Stress in Rainbow Trout (Oncorhynchus mykiss) |
| MA Fang, LIU Zhe*, KANG Yu-Jun, QUAN Jin-Qiang |
| College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Rainbow trout (Oncorhynchus mykiss) is a typical cold-water fish, which has poor tolerance to higher water temperature. With the global warming, the impact of heat stress on rainbow trout is becoming more and more serious. To further understand the mechanism of heat shock proteins (Hsps) in rainbow trout during heat stress, in this study, 36 Hsp40 family genes were identified by bioinformatics analysis of rainbow trout genome database and RNA-seq data. The physiochemical properties, Phylogenetic, gene structure and gene expression profile of rainbow trout Hsp40 family gene were systematically analyzed at the whole gene level. The results showed that these genes were divided into three subtypesⅠ,ⅡandⅢ according to their structural similarities. The number of amino acid ranged from 190 to 760, and the theoretical pI distribution ranged from 5.01 to 9.09. Gene structure analysis showed that the Hsp40 family genes of rainbow trout had only 1 exon. Subcellular localization analysis showed that the Hsp40 gene was mainly expressed in cytoplasm, nucleus and mitochondria, and the secondary structure was mainly alpha-helix and irregular curl. The expression profile of Hsp40 in rainbow trout was analyzed by RNA-seq data. The results showed that 8 and 10 Hsp40 genes were up-regulated in liver and head kidney, respectively, and in total of 7 Hsp40 genes were up-regulated in both tissues. The significant expression regulation of Hsp40 family genes after heat stress indicates that Hsp40 genes are involved in the heat stress of rainbow trout, which provides theoretical basis and basic data for studying the regulation mechanism of Hsp40 family gene members against heat stress.
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Received: 11 March 2019
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Corresponding Authors:
liuz@gsau.edu.cn
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