Abstract：In order to study yellow stripe like from Malus xiaojinensis(MxYSL5) gene expression and regulation, the regulatory sequence(891 bp) of MxYSL5 gene was cloned by Tail-PCR. Silico analysis of sequence suggested that the sequence contained several typical cis-acting elements, including multiple light-responsive elements(LREs), ethylene-responsive element(ERE), gibberellins(GAs)-responsive element(GARE, P-box), TATA-box and CAAT-box. The 891 bp promoter sequence upstream of translation start site of MxYSL5 was cloned and designated as MxYP5-GFP. MxYP5-GFP was constructed and transformed into onion(Allium cepa) epidermal cells by particle bombardment. The result showed that MxYP5 could drive the transient expression of GFP protein in onion epidermal cells, can be used to show the expression and regulation state of MxYSL5.
