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2025年8月15日 星期五
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肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析
张跟喜1,丁馥香2,是燕萍3,王金玉1,张李俊3,张丽3,施会强3
1. 扬州大学动物科学与技术学院
2. 山西省农科院畜牧兽医研究所
3.
Polymorphisms in Exon 1 of the Myostatin Gene (MSTN) and Its Relationship with Growth Traits in Bian Chicken
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摘要 根据GenBank发表的鸡MSTN基因序列针对外显子1设计2对引物,采用PCR-SSCP技术检测边鸡(Gallus gallus)及3个对照群体(京海黄鸡(Gallus gallus)、尤溪麻鸡(Gallus gallus)和AA鸡(Gallus gallus))的单核苷酸多态性,并与边鸡的生长性状进行相关性分析。结果表明:MYOE1-1扩增片段不存在多态性。MYOE1-2扩增片段存在多态性,在边鸡、京海黄鸡和尤溪麻鸡中都检测到6种基因型(AA、AB、BB、BC、AC和CC),而在AA鸡中只检测到4种基因型(AB、BC、AC和CC)。克隆测序表明,总共检测到3个多态位点(G2100A、G2109A、C2244G),这3个突变均未导致氨基酸的改变。最小二乘分析表明,在6-8和12周龄时,BB基因型个体的体重显著高于AA基因型个体(P<0.05)。在14周龄时,BB基因型个体的体重显著高于AA和AC基因型个体(P<0.05)。在16-18周龄时,BB基因型个体的体重显著的高于其余5种基因型的个体(P<0.05)。研究结果初步表明:MSTN基因可能是影响边鸡生长性状的一个主效基因或是与之存在紧密遗传连锁的一个标记。
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张跟喜
丁馥香
是燕萍
王金玉
张李俊
张丽
施会强
关键词 肌肉生长抑制素边鸡PCR-SSCP生长性状    
Abstract:In order to lay a foundation for Marker-assisted Selection (MAS) for Bian chicken's growth traits, single nucleotide polymorphisms (SNPs) of exon 1 of the myostatin(MSTN) gene were detected in Bian chicken(Gallus gallus) and three reference chicken (G. gallus) populations (the Jinghai, Youxi and Arbor Acre chickens) by PCR-SSCP. And the relationship with growth traits in the Bian chicken was analysed. Results showed that the Bian, Jinghai and Youxi chickens all displayed six genotypes (AA, AB, BB, BC, AC and CC), while only four genotypes (AB, BC, AC and CC) were detected in the Arbor Acre chicken. Sequencing revealed three mutations (G2 100A, G2 109A and C2 244G) of the MSTN gene. Chickens of BB genotype had significantly higher body weight than those of AA genotype (P<0.05) from 6 to 8 week of age and 12 week of age. Chickens of BB genotype had significantly higher body weight than those of AA and AC genotypes at 14 week of age (P<0.05). Chickens of BB genotype had significantly higher body weight than those of the other five genotypes from 16 to 18 week of age (P<0.05). These results preliminarily show that the MSTN gene is either a major gene that influences the body weight traits of the Bian chicken or a molecular marker in close linkage with them.
Key wordsMSTN    Bian chicken    PCR-SSCP    Growth traits
收稿日期: 2010-04-13     
通讯作者: 王金玉   
引用本文:   
张跟喜1,丁馥香2,是燕萍3,王金玉1,张李俊3,张丽3,施会强3. 肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析[J]. , 2011, 19(1): 122-127.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2011/V19/I1/122
 
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