Abstract:Real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of thyroglobulin (TG) and thyroid peroxidase (TPO) genes from the thyroid tissue of castrated and uncastrated Jinhua pigs at the ages of 60, 90 and 120 days. Results showed that the expression levels of TG and TPO mRNA in castrated Jinhua pigs at 90 days was significantly higher than that of other days (P<0.05), while the expression level of TG in uncastrated pigs at 120 days was significant lower than those at 60 and 90 days (P<0.05), but no significant difference in TPO expression level among ages (P>0.05). The comparison of TG and TPO mRNA between castrated and uncastrated pigs was different significantly at 60, 90, and 120 days (P<0.05). The results suggested that there may have been a positive relationship of the expression levels of the TPO and TG mRNA with the weight gain and intramuscular fat content in pig after castration.