Abstract：Using phage display technique we constructed a library of repertoire immunoglobulin from mouse immunized with SS. The heavy-chain and light－chain variable region gene (VH and VL) repertoire of immunoglobulin were amplified individually from the spleen cell RNA by RT－PCR and joined by a DNA linker encoding (G1y4Ser)3 as a single chain (ScFv) DNA fragment with overlap extension. These fragments were cloned into the phagemid pCANTAB5E and the recombinant phagemid was transformed to susceptible E.coli TGl by electroporation.In the presence of helper phage M13KO7, the ScFv fusion protein were displayed on the surface of recombinant phages. The phage antibody library was generated and its size was determined to be 9.3×107,these results are bases to further phase antibody library construction
