Abstract:A totle of sixty one-day-old Yellow brioler chickens were alloted into treatment and control groups. The treatment group was fed with the diet supplemented with 3% conjugated linoleic acid(CLA) for 48d, while control group was fed with the diet supplemented with 3% rapeseed oil. Chickens were slaughtered in each group at the age of 49 d, and abdominal adipose tissue was sampled. The total RNA was extracted from adipose tissue to measure the abundance of the chicken growth hormone receptor(cGHR), insulin-like growth factor 1(cIGF-1), insulin-like growth factor Ⅰreceptor(cIGF-ⅠR), peroxisome proliferator-activated receptor gamma(cPPARγ), cAdiponectin and cAdipoⅠR mRNA by RT-PCR using β-actin as an internal standard. Results showed that the CLA decreased the abdominal fat index by 20.93%(P<0.05). CLA down-regulated the relative abundance of cGH-R mRNA and PPARγ mRNA in abdominal adipose tissue by 24.74%(P<0.05)and 66.52 %(P<0.01)respectively. However, no differences in the relative abundance of cIGF-1, cIGF-ⅠR, cAdiponectin, and cAdipoⅠR mRNA in abdominal adipose tissue between CLA treatment group and control group were found(P>0.05). The data demonstrated that CLA inhibitted abdominal fat deposition preadipocytes via down-regulation of PPARγ, in broiler chicken may determine by decreasing the GH-R available for GH, and by inhibiting the differentiation of but independent of IGF and (or) GH-IGF pathway or adiponectin action.
周杰 陆春瑞 郑智勇 李 舜 朱凤桥. 日粮共轭亚油酸对黄羽肉鸡腹脂沉积的影响及机理研究[J]. , 2008, 16(2): 0-.
ZHOU Jie LU Chunrui ZHENG Zhi-Yong LI Shun ZHU Feng-Qiao. Effect of dietary Conjugated linoleic acid (CLA) on Abdominal Fat Deposition in Yellow Broiler Chickens and its possible Mechanism. , 2008, 16(2): 0-.