Impact of TL-DNA on Content of Ginsenosides in Ginseng Hairy Roots and Cloning and Sequence Analysis of rolC Gene
Zhao Shoujing1 Li Changyu2 Qian Yanchun1 Shi Jing1 Liu Qingchang3
（1.College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China; 2. Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China; 3. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China）
摘要摘要：通过发根农杆菌?穴Agrobacterium rhizogenes ?雪与人参?穴Panax ginseng C. A. Mey?雪根外植体共培养，用直接接菌方法诱导出人参发根。培养4周后人参发根的总皂苷含量达到栽培3年生人参皂苷含量的水平，单体皂苷Rb1含量明显提高，说明TL-DNA具有影响人参皂苷生物合成的能力。根据Slightom等RiA4TL-DNA序列分析结果，利用PCR方法从人参发根中扩增并克隆了影响人参皂苷合成的基因rolC。与已发表序列相比较，核苷酸序列的同源性为99.9%。
Abstract：Abstract: Ginseng （Panax ginseng C.A.Mey）hairy roots were induced from the root explants of ginseng by the co-culture of Agrobacterium rhizogenes and ginseng roots with the method of direct inoculation. The content of ginsenosides in ginseng hairy roots after 4 weeks culture basically reached the level of that in 3-year-old cultivated ginseng roots, and the content of single ginsenoside-Rb1 had an evident increase. The result indicated that TL-DNA had a function of influencing the biosynthesis of ginsenosides. Based on the analytical results of RiA4TL-DNA sequence, rolC gene affecting the synthesis of ginsenosides was amplified and cloned from the ginseng hairy roots by PCR method. Comparison with previously published nucleotide sequence showed that the homology was 99.9%.
赵寿经1 李昌禹2 钱延春1 石 晶1 刘庆昌3. 影响人参发根皂苷含量基因rolC的克隆与序列分析[J]. , 2003, 11(3): 249-252.
Zhao Shoujing1 Li Changyu2 Qian Yanchun1 Shi Jing1 Liu Qingchang3. Impact of TL-DNA on Content of Ginsenosides in Ginseng Hairy Roots and Cloning and Sequence Analysis of rolC Gene. , 2003, 11(3): 249-252.
