Abstract：Melanocortin- 4 receptor (MC4R) is a member of the superfamily of G protein coupled receptors (GPCRs), which affects body weight, energy homeostasis and food intake in human and mouse. In this study, the Asp298Asn polymorphism of MC4R gene in Laiwu, Yorkshire×Laiwu and commercial crossbred pig populations was investigated using PCR-RFLP method, and, the relationship of this mutation with backfat thickness was analyzed. The results indicated that only genotype 11 exist in 33 individuals of Laiwu pigs, whereas in Yorkshire×Laiwu and commercial crossbred populations, three genotypes of 11, 12 and 22 were detected. The distributions of allele and genotype frequencies in Yorkshire×Laiwu and commercial crossbred populations were similar with the frequency of allele 1 being higher than that of allele 2. All of the three populations were at Hardy-Weinberg equilibrium with respect to this mutation site（P>0.05）. In commercial crossbred pigs, individuals with genotype 22 had significant higher backfat thickness than the ones with genotypes 12 and 11 (P < 0.05). This study provide evidence that the Asp298Asn polymorphism of MC4R is associated with backfat thickness in commercial crossbred pigs with western pigs as parental lines, and therefore, can be used as a DNA marker for pig breeding in such kind of populations.
姜运良 杨晓慧 刘源 张宁波 武英. 猪MC4R基因Asp298Asn位点的多态性及其与商品猪背膘厚的关系[J]. , 2008, 16(3): 0-.
Yun-Liang JIANG Xiao－Hui YANG Yuan LIU Ning－Bo ZHANG Ying WU. Polymorphism of MC4R Asp298Asn Site and its Relationship with Backfat Thickness in Commercial Pigs. , 2008, 16(3): 0-.
