Abstract：In this study, the in-vitro maturation (IVM) and in-vitro fertilization (IVF) of ovine oocytes with inhibitor, BL-I, was investigated. Oocytes were cultured in vitro (IVC)among different durations after being inhibited for 8h. Results: MⅡ rate of the 8h IVC group was significantly lower than the other groups. There were no significant differences of the maturation rates among the 16h、20h IVC groups and the control one; the maturation rate (70.83%) of the 24h IVC group was higher than the control one (66.18％) ,but there were no significant differences between them (P>0.05). There were significant differences of the blastocyst rates among the 16h、20h IVC and control groups . Conclusion: The effect of inhibition by BL-I on IVM of ovine oocytes is reversible; and the inhibition treatment with BL-I on ovine IVM, can not improve the fertilization in-vitro and developmental competence of embryos.
