Abstract：microRNAs (miRNAs), a class of small ~22-nucleotide-long noncoding RNAs, emerge as key post-transcriptional regulators of gene expression in eukaryotic organisms and involve in a variety of cellular processes, such as cell proliferation, cell differentiation and apoptosis. In silico analysis of the tumor necrosis factor-α (TNF-α) and miR-369 showed that the 3'UTR region of porcine(Sus scrofa) TNF-α adenylate/uridylate-rich elements(AREs) contained two putative miR-369 target sites. The porcine TNF-α 3'UTR region was constructed into the dual-luciferase reporter vector, transfected in the porcine iliac artery endothelial cell (PIEC cell) line to measure the luciferase activity by dual-luciferase reporter assay. The results indicated that the luciferase activity of miR-369 mimics group was significantly lower than that of the control group (P<0.05). Nonetheless, there was not a significant differences between the inhibitors and the control groups (P=0.752). Our results demonstrated that the TNF-α should be the target gene of miR-369 and provides evidence for further research of post-transcriptional mechanisms of TNF-α which affect fat deposition
