Abstract：KANADI(KAN) genes are involved in controlling the dorsal-ventral polarity of plant lateral organs in Arabidopsis. For further research on the molecular mechanism of plant leaf polarity establishment, all members of KANADI family in Nicotiana benthamiana were cloned by RT-PCR method and their organ expression profile was examined. The Results showed that there were 8 KANADI gene family members in N. benthamiana genome, their encoding proteins shared around 70%~93% similarity to the homologs in Solanaceae and bear the specific conserved region and GARP domain that were found in plant KANADI protein family. Bioinformatic analysis indicated that NbKAN were non-secreted hydrophilic proteins without identifiable transmembrane structures. The results of Real-time qRT-PCR showed that NbKAN genes were present in all plant organs examined and they displayed different expression patterns.Therefore, the experiment provides the important basis for further study the relationships of KANADI gene function and other gene family.
