Abstract:In order to confirm the popularity and mechanism of female fertility of triploid lilies(Lilium), Longiflorum-Asiatic lily Brindis(3x) and Pavia(3x) were used as maternal parents to cross with Asiatic lily Monte Negro(2x) and Tresor(4x) as male with normal pollination in the present paper. 21 fruits were obtained from 3 combinations, which were mostly developed well, while, the number of developmental embryos and endosperm from different fruits ranged from 6~42. Finally, 97 seedlings were obtained after embryo rescue within these fruits. The maternal parents Brindis and Pavia and their seven progenies were analyzed by using genomic in situ hybridization(GISH). The results showed that the seedlings were true progenies from the hybridizations. All of them were aneuploid whose chromosome numbers ranged from 36 to 63. The progenies contained different recombinant chromosomes from their two maternal parents. It further supports that, regardless of male sterility, triploid lilies can be used as maternal parents to cross with appropriate males to produce aneuploid progenies. Due to aneuploids may cause great variation in morphological and physiological traits, it is expected that allotriploid lilies can be used to create more variable new lily forms with foreign genes.