Single Nucleotide Polymorphisms(SNPs) of Carboxypeptidase A1 Gene (CPA1) Segments and Their Association with the Growth Traits of Grass Carp (Ctenopharyngodon idella)
Abstract：Carboxypeptidase A (EC 3.4.16) includes a type of digestive enzymes that hydrolyzes the C-terminal aromatic or aliphatic amino acids of a protein. Single nucleotide polymorphism (SNP) analysis of genes functionally related to the growth traits of grass carp (Ctenopharyngodon idella) can provide useful information for grass carp molecular breeding. In this study, genomic fragments of carboxypeptidase A1 (CPA1) gene were amplified based on the sequences of two available contigs in the EST-SNP database of grass carp. Two SNP mutations were identified after sequencing the PCR prodcts, C+412A and A36C, which were located in exon 5 and intron 3 of the CPA1 gene, respectively. Regarding the two SNPs, the genotype and gene frequency of 296 grass carps were further assayed with the method of SnaPshot. The frequencies of AA, AC and CC were 26.7%, 52.0% and 21.3% at the A36C site, but 15.5%, 40.5% and 43.9% at the C+412A site, respectively. A general linear model was used to do the correlation analysis between the CPA1 gene SNPs and growth traits. The three different genotypes of the A36C site were significantly associated with body weight and eye distance (P<0.05). At this site, the genotype of AA significantly showed a higher value on body weight, body length, body width and eye distance compared to the genotype of CC (P<0.05). Although the C+412A site showed no significant correlation with body weight (P<0.05), the genotypes of CC and AC differed significantly for body weight and length before anus (P<0.05). Moreover, the genotype of CC always showed a greater values than that of AC or AA for six growth characters. Five diplotypes consisted of the two SNPs were different on body weight. For example, the body weight of diplotypes D3 and D5 differed significantly (P<0.05). Diplotype D3 showed a significant higher value of body weight, body length, body width and the ratio of body length/head length differences compared with genotype D8 (P<0.05).These results indicate that the CPA1 gene can be a candidate gene that can be useful for grass carp molecular breeding.
曹婷婷1,白俊杰1,于凌云樊佳佳1. 草鱼羧肽酶A1基因(CPA1)部分片段的单核苷酸多态性(SNP)多态性及其与生长性状的关联分析[J]. , 2012, 20(3): 301-307.
1, Bai-Junjie1, 1. Single Nucleotide Polymorphisms(SNPs) of Carboxypeptidase A1 Gene (CPA1) Segments and Their Association with the Growth Traits of Grass Carp (Ctenopharyngodon idella). , 2012, 20(3): 301-307.
