Both Caspase family and Bcl-2 family were involved in regulating apoptosis in HL-60 cells induced by Bacillus thuringiensis Bt9875 insecticidal crystal proteins
Abstract：Abstract: To study the effect of Caspase and Bcl-2 families on apoptosis HL-60 cells induced by insecticidal crystal proteins from Bacillus thuringiensis Bt9875. The activation of Caspases and the effect of Caspase inhibitors on apoptosis of HL-60 cells were detected. The cleavage of poly ADP-ribose polymerase (PARP), Bcl-2/Bax regulation and the release of cytochrome C were observed by Western blot analysis. Insecticidal crystal proteins from Bacillus thuringiensis Bt9875 can activate Caspase-3, 8 and 9. Moreover, the cell death can be significantly suppressed by family Caspase inhibitor (Z-VAD-FMK), Caspase-3 inhibitor (Z-DEVD-FMK) and Caspase-9 inhibitor (Z-LEHD-FMK). Futhermore, up-regulation of Bax and down-regulation of Bcl-2 have also been detected by Western blot in HL-60 cells which treated with Bt9875. The results suggest that the progression of cell death induced by Bt9875 was mediated by both Caspase family and Bcl-2 family. The mitochondria pathway plays an important role on apoptosis of HL-60 cells induced by insecticidal crystal proteins from Bacillus thuringiensis Bt9875.
