Abstract：A method was established to distinguish milk powder and bovine or ovine meat and bone meal in feedstuffs, which were all ruminant-derived. Milk powder in feedstuffs was washed and removed by water and 0.5% sodium hypochlorite solution, subsequently, the ruminant-derived materials were identified using PCR according to 16S rDNA sequence. The detected limitation of bovine or ovine in feedstuffs with 25%, 50% and 75% milk powder concentrations were 2%, 0.5% and 0.1%, respectively. Taken altogether, this method is a simple and easily grasped way for identification and discrimination of the bovine or ovine-derived meat and bone meal and milk powder in feedstuffs.
