Abstract：The coding sequence (CDS) of CAAT/enhancer-binding protein α (C/EBPα) gene was obtained from T-C/EBPα plasmid by enzyme digestion. The recombinant C/EBPα was expressed in Escherichia coli with IPTG induction and purified by Glutathione Sepharose 4B affinity chromatography. The purified C/EBPα was used as immunogen to immunize the rabbit. The titer of the generated antiserum was detected to be 1∶12800 by ELISA. Western blotting result showed that the antiserum had high specificity. Expression characteristics showed that C/EBPα expressed abundantly in muscle stomach, glandulose stomach, kidney, intestine, liver, heart, fat, spleen and lung respectively while little in muscle. The antiserum was meanwhile used to analyse the C/EBPα expression characteristics between fat and lean line roosters，which showed that C/EBPα expressed higher in lean line rooster livers.
