Abstract：Polymerase chain reaction (PCR) is perspective for detection of animal derived feedstuffs. In order to avoid safety risks deriving from bovine spongiform encephalopathy and detect fish-derived material efficiently by PCR method, specific primers were designed according to mitochondria 16S rRNA sequence. DNeasy Tissue Kit of Qiagen was applied to extract nucleic acid. Specificity detection results showed primer NC_Fish2480/2501F, NC_Fish2565/2586R had no cross-reaction with bovine, ovine, porcine and chicken derived material. Sensitivity results showed that the detection limitation was 0.1% fish derived material. This study provided a method for PCR detection fish-derived material in ruminant feedstuffs.
