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2025年8月19日 星期二
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拟南芥受伤和接种根癌农杆菌GV3101对转录的影响
肖卫民1,3,赵名琛1,3,邹敏2,苏承刚1,3,杜小兵1,3,谭远德2,张兴国4
1. 南方山地园艺学教育部重点实验室
2.
3. 南方山地园艺学教育部重点实验室
4. 西南大学园艺园林学院
Impact of Wounding or Agrobacterium tumefaciens GV3101 on Differential Transcription of Arabidopsis thaliana Inflorescence Stalk
2, 2, 2, 2, 2,
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摘要 根癌农杆菌(Agrobacterium tumefaciens)侵染植物会引起感染细胞的基因转录发生变化。明确其间的基因转录模式,有助于揭示农杆菌的致病机理和改善农杆菌介导的遗传转化技术。本研究采用RAM(ranking analysis of microarray data)统计方法,分析拟南芥(Arabidopsis thaliana)受伤和接种农杆菌GV3101后第3小时至第6天的转录组变化,采用MapMan软件分析所得差异转录基因的功能类别与分布。结果显示,农杆菌GV3101导致4 651个基因差异转录,而受伤时有3 885个基因的转录发生改变,两者之间有1 045个相同基因。接种农杆菌GV3101后拟南芥基础代谢相关的差异转录基因数目减少,并且使胁迫途径中植物致病相关蛋白(pathogenesis-related proteins, PR proteins)的编码基因转录变化显著,其中37个NB-LRR (nucleotide-binding site, leucine-rich repeat)型抗病蛋白(resistance proteins, R proteins)的编码基因转录水平明显下降。受伤时只有6个NB-LRR型蛋白的编码基因转录下调。研究结果表明,接种根癌农杆菌GV3101后拟南芥细胞的转录模式明显改变,抗病能力受到抑制,有利于农杆菌T-DNA的整合和致病。
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肖卫民
赵名琛
邹敏
苏承刚
杜小兵
谭远德
张兴国
关键词 拟南芥受伤花梗根癌农杆菌GV3101转录组    
Abstract:Gene expression or transcription profile of plant cells changes after inoculation with Agrobacterium tumefaciens. To understand the dynamic transcription and the modulation of metabolism pathways in plant infected with Agrobacterium tumefaciens, transcriptomic data of Arabidopsis thaliana inflorescence stalks from 3 h and 6 d after wounding or inoculation with GV3101 were analyzed by performing the statistical method RAM and the pathway analysis program MapMan. The results showed that 4 651 genes were differentially transcribed in the inflorescence stalks after inoculation with GV3101 while 3 885 genes changed in transcription after wounding, among which 1 045 genes were common. When inoculated with GV3101, the number of differently transcribed genes related to primary metabolism was reduced in comparison with the reference. Subcategorization of genes in stress category showed that 60 genes coding for PR proteins (pathogenesis-related proteins) were transcribed differentially after inoculation with GV3101, among which 37 genes coding for NB-LRR (nucleotide-binding site, leucine-rich repeat) disease resistance proteins were down-regulated. While in reference stalks, only 18 PR proteins-encoding genes were differentially transcribed, among which 6 genes coding for NB-LRR proteins were transcribed negatively. These results suggested that Agrobacterium GV3101 modulates gene transcriptional profile in Arabidopsis thaliana and suppresses disease resistance of host plant cells, which might facilitate the successful integration of T-DNA into host genome.
Key wordsArabidopsis thaliana    Wounded inflorescence stalk    Agrobacterium tumefaciens GV3101    Transcriptome
收稿日期: 2012-09-17     
通讯作者: 张兴国   
引用本文:   
肖卫民1,3,赵名琛1,3,邹敏2,苏承刚1,3,杜小兵1,3,谭远德2,张兴国4. 拟南芥受伤和接种根癌农杆菌GV3101对转录的影响[J]. , 2013, 21(5): 537-545.
2, 2, 2, 2, 2,. Impact of Wounding or Agrobacterium tumefaciens GV3101 on Differential Transcription of Arabidopsis thaliana Inflorescence Stalk. , 2013, 21(5): 537-545.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2013/V21/I5/537
 
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