Abstract：In order to increase the frequency of wheat haploid embryo formation through wheat × maize, we implied two method of hormone treated: Different volume concentrations of DMSO in 100mg/L 2, 4-D (treatment 1) and different hormone treatment combinations (treatment 2). The results showed that different volume concentrations of DMSO and different hormone treatment combinations had difference on the frequency of embryo formation. In treatment 1, the optimal volume concentration of DMSO in 2, 4-D is 3.0%(17.0%), moreover the frequencies of haploid embryo formation in other volume concentrations of DMSO are all higher than in control (9.7%).The frequency of embryo formation of different hormone treatment combinations was that 2,4-D+3.0% DMSO single treatment (7.3%)﹥2,4-D twice treatments (6.9%)﹥2,4-D and 2,4-D+3.0% DMSO combined treatment (5.5%)﹥2,4-D single treatment (2.9%).
王成社 闫林 . 提高小麦×玉米杂交产生单倍体胚频率的研究[J]. , 2007, 15(2): 0-.
. Study on Increasing Frequency of Wheat Haploid Embryo Production through Wheat×Maize. , 2007, 15(2): 0-.
