摘要根据白菜花粉特异的多聚半乳糖醛酸酶基因BcMF9的全长序列设计引物，通过PCR直接扩增的方法从结球甘蓝（Brassica. oleracea var. capitata）和芥蓝（B. oleracea var. alboglabra）中克隆得到BcMF9的同源基因BoMF9（BoMF9c和BoMF9l）。生物信息学分析和系统树构建发现BoMF9c和BoMF9l具有花粉特异表达的多聚半乳糖醛酸酶基因的序列特征。不同组织的表达特征分析发现它们分别在结球甘蓝和芥蓝的花蕾中特异表达。推测BoMF9c和BoMF9l可能在花粉萌发和花粉管伸长中起作用。
Abstract：BoMF9 (BoMF9c and BoMF9l) gene, homologous to the BcMF9 gene encoding pollen-specific polygalacturonase of Chinese cabbage，were cloned from Brassica oleracea var. capitata and B. oleracea var. alboglabra by PCR amplification. Sequence analysis and phylogenetic analysis revealed that the deduced amino acids sequences of BoMF9c and BoMF9l contained characteristic sequence of polygalacturonase expressed in pollen. RT-PCR analysis again showed that BoMF9c and BoMF9l were expressed in flower buds but not other tissues. These two genes may act as polygalacturonases involving in pollen development.
