Abstract：Lipoprotein lipase（LPL）is a key enzyme for the metabolize of circulating triacylglycerols. It is considered to be the major candidate gene affecting fat thickness and intramuscular fat content etc. In this study, PCR-RFLP was applied to analyze the polymorphism of LPL gene in 238 cattle that comprised of six bovine breeds, namely Angus、Hereford、Chinese simmental、Charolais、 Luxi cattle、simmental×Mongolia cattle. A 378 bp-long PCR product was digested with MspⅠ. The result showed that BB genotype had one substitution mutation A/G in 263bp. All six breeds were at Hardy-Weinberg equilibrium（P>0.05）.Except Angus, the polymorphism information contents of other breeds were moderate polymorphisms（0.25
