Abstract：The plant expression vectors pCAMBIA1301PMI and pBIPMI were constructed by substituting Escherichia coli phosphomannose- isomerase (PMI) gene for hpt gene of pCAMBIA1301 and GUS gene of pBI121. Epicocyl explants of ‘Xuegan’ sweet orange were inoculated with EHA105- pCAMBIA1301PMI and EHA105- pBIPMI and subsequently selected on medium supplemented with a combination of 25 g•L-1 mannose and 5 g•L-1 sucrose as a carbon source. The transformation efficiency rate was 27.7% when transformed by pCAMBIA1301PMI and 12.7% by pBIPMI .Genetic transformation was confirmed by Chlorophenol Red assay and PCR. A new method for obtaining transgenic ‘Xuegan’ plants was developed using PMI/mannose selection system.
曾黎辉 徐海峰 王会全 吴少华 朱艺萱. PMI基因为选择标记植物表达载体的构建及在雪柑转基因中的应用[J]. , 2008, 16(5): 0-.
. The construction of plant expression vectors with PMI gene as selection marker and utilization in transformation of ‘Xuegan’. , 2008, 16(5): 0-.
