Abstract：In order to study the function of a putative interferon responsive gene (Ifrg15), the genomic DNA of Kunming White mouse(Mus musculus) was purified and used as the PCR template for amplifying the Ifrg15 coding region. The PCR product was cloned into pGEM-T vector and confirmed by DNA sequencing. The Ifrg15 fragment was subcloned into pET-41(c) vector by EcoR Ⅰ and Xho Ⅰ double-digestion. Escherichia coli BL21(DE3) transformants were then selected and tested by IPTG induction for high level expression of GST&6xHis-Ifrg15 fusion protein. The results showed that mouse Ifrg15 gene was successfully cloned and expressed in the prokaryotic expression system. By using denaturing condition in the affinity chromatographic step, a decent level of purification of GST&6xHis-Ifrg15 fusion protein was achieved.
