Abstract：With more and more appearence of meat adulteration, one simple, high-effency and quick detection method is urgent which compares to tradional ones with expensive, low-accuracy and low-efficiency. In this study, adulteration detection with molecular biology techniques was researched. A pair of highly specific primers for pork, beef and sheep were obtained, respectively, by trational PCR screening and verification. The Real-time fluorescence quantitative PCR results of pork, beef and sheep with 3 primer combinations showed that the detection limits were 1.69 copy, 1.52 copy and 17.5 copy, respectively. The 3 primer combinations were then developed into detection kits for pork, beef and mutton. The kits developed in this study identified pork, beef and mutton effectively when they were used to detect several deep-processed meat products in market. The kit is simple, which shows a good application prospect in field use.
