Abstract：Based on quantitative traits loci (QTL) mapping, porcine uncoupling protein 3 (UCP3) gene was chosen as a candidate gene for pig fat deposition and meat quality traits. Partial coding region of UCP3 gene was sequenced and there was one single nucleotide polymorphisms (cSNP) at 395 bp. This mutation was G→A and it resulted in the amino acid change from glycine to arginine. This site was also recognized by restriction endonuclease SmaⅠ. The polymorphism of the UCP3 SmaⅠwas analyzed with PCR-RFLP among 186 Large white × Meishan F2 progeny. The genotypes of UCP3 SmaⅠwere AA, AB and BB. The frequency of A allele was 0.56 and B was 0.44. With statistical analysis, it was found that the SmaⅠ polymorphism in the F2 population was significant associated with backfat thickness at thorax-waist and at buttock, intramuscular fat, drip loss rate and water holding capacity. The function of UCP3 SmaⅠmainly showed additive effect. The genotype AA reduced back-fat thickness and water drip loss rate, and increased water holding capacity. Meanwhile, it also decreased the intramuscular fat content .
任亮;朱宝芹;韩丹;张轶博;宋惠娟;曾瑞霞;苏玉虹. 猪UCP3基因多态性及其与脂肪沉积、肉质性状的相关分析[J]. , 2006, 14(5): 652-656.
REN Liang;ZHU Bao-qin;HAN Dan;ZHANG Yi-bo;SONG Hui-juan;ZENG Rui-xia;SU Yu-hong. Polymorphism of Porcine Uncoupling Protein 3 (UCP3) and Its Association with Fat Deposition and Meat Quality . , 2006, 14(5): 652-656.
