Abstract:To investigate the serotype distribution, antimicrobial susceptibility and resistant mechanisms of Salmonella isolated from chickens(Gallus domesticus), 110 Salmonella from Beijing and Shanxi were studied. The results of serotyping indicated that S.enteritidis(76.58%) was the predominant serotypes. The susceptibility of 110 Salmonella isolates to 13 kinds antimicrobials was determined by microbroth dilution method. The highest resistant rate was observed by polymyxin E(41.82%), followed by sulfisoxazole(31.82%), ampicillin(29.09%) and doxycycline(20.91%). Resistant rates to other antimicrobials were under 20%. Resistant mechnisms of isolates were detected, and the results showed that integronⅠmainly harbored the resistance genes to aminoglycosides, sulfonamides, lincosamides, which might play a critical role in spresding drug resistance. This study provides basic data for prevalence investigation of Salmonella from chickens and the rational use of antimicrobials for Salmonella infection control in veterinary practice.