Cloning and Expression Analysis of Tudor Domain-containing Protein 3 (TDRD3) Gene in Strongylocentrotus intermedius
LI Hao-Wen, ZHANG Xi-Ying, WANG Heng*, LI Jia-Lin, LI Xi-Yu, DING Jun, CHANG Ya-Qing
Key Laboratory of Seawater Enhancement and Aquaculture in North China, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
Abstract：Germline factors not only regulate germ cell development but may also play important roles in somatic cells. The previous study found that the Tudor domain-containing protein 3 (TDRD3) gene, a member of the TDRD family of germline factors, is significantly upregulated in the gonads of Strongylocentrotus intermedius, which is rich in high concentration of polyunsaturated fatty acids. To investigate the roles and regulatory mechanisms of TDRD3 in non-germline cells and embryonic development, S. intermedius was used as the material, bioinformatics and quantitative molecular approaches were applied to identify and characterize the TDRD3 gene, and to analyze its sequence features as well as its expression patterns across different tissues and developmental stages. The results showed that the full-length cDNA of the TDRD3 gene (GenBank No. PP496300.1) was 4 361 bp, containing a 2 814 bp open reading frame that encoded a protein of 937 amino acids. The predicted protein was hydrophilic and unstable, with 1 conserved Tudor domain. Expression analysis demonstrated that TDRD3 was broadly expressed in various adult tissues and throughout embryonic development in S. intermedius, suggesting that it played essential roles across the organism's life cycle. Notably, TDRD3 expression was significantly elevated in the ovary and during embryogenesis, indicating its potential involvement in gonadal growth and development. This study provides a theoretical foundation for elucidating the molecular mechanisms underlying gonadal and embryonic development in S. intermedius, and offers insights that may contribute to the sustainable aquaculture of this species.
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