The Effect of Resveratrol on Sperm Capacitation In vitro and Early Embryo Development in Bovine (Bos taurus)
LI Xiao-Xia1,2,*, CAO Ping-Hua1, WANG Dou-Dou1, QU Pei-Dong1, XU Zhi-Qian1,2, YU Xue-Li1,2, LI Ying-Hua1,2
1 College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China; 2 Henan Provincial Key Laboratory for Grass-Feeding Animal, Luoyang 471023, China
Abstract：An excess production of reactive oxygen species (ROS) during sperm capacitation in vitro will decrease sperm quality, and affect early embryonic development. Resveratrol is an effective anti-oxidant to protect sperm. In this study, the effect of resveratrol was evaluated on sperm capacitation in vitro and early embryo development in bovine (Bos taurus). The frozen-thawed semen from Holstein bulls was used in sperm capacitation medium supplementing with 0, 10, 50 100 and 500 μmol/L resveratrol, subsequently incubated by swim-up method for 1 h. This study was to evaluate the effects of resveratrol on bovine sperm progressive motility, acrosome integrity, mitochondrial activity, plasma membrane integrity, capacitation status and the capacity of early embryo development after in vitro fertilization. These results showed that compared with the control group (0 μmol/mL), the supplementation of 50, 100 μmol/L resveratrol in sperm capacitation medium could significantly increase sperm progressive motility, the percentage of sperm with acrosome integrity, high mitochondrial membrane potential and plasma membrane integrity in bovine after in vitro capacitation (P<0.05); and it significantly increased the percentage of the B pattern sperm. Both of chlortetracycline (CTC)fluorescent staining and coomassie staining could be used to assess acrosome integrity of bovine sperm, and there was no significant difference between the 2 methods (P>0.05). The results of in vitro fertilization showed that compared with the control group (0 μmol/mL), the supplementation of resveratrol at 100 μmol/L in the capacitation medium resulted in a higher cleavage rate and blastocyst rate (P<0.05). This result showed that adding 100 μmol /mL resveratrol to capacitation medium could significantly improve sperm function of bovine after in vitro capacitation, increase its capacitation rate, so that the cleavage and blastocyst rate were significantly increased after in vitro fertilization. This study could help to optimize in vitro fertilization procedures and accelerate the efficiency of in vitro embryo production.
李晓霞, 曹平华, 汪豆豆, 曲培栋, 徐志谦, 禹学礼, 栗颖华. 白藜芦醇对牛精子体外获能效果及早期胚胎发育的影响[J]. 农业生物技术学报, 2024, 32(8): 1836-1843.
LI Xiao-Xia, CAO Ping-Hua, WANG Dou-Dou, QU Pei-Dong, XU Zhi-Qian, YU Xue-Li, LI Ying-Hua. The Effect of Resveratrol on Sperm Capacitation In vitro and Early Embryo Development in Bovine (Bos taurus). 农业生物技术学报, 2024, 32(8): 1836-1843.
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