Development of KASP Assays of 9 Genetic Defects in Simmental Cattle (Bos taurus)
PEI Fen1, ZHANG Ke1, KHAN Md Yousuf Ali1, SI Jing-Fang1, DAI Dong-Mei1, OU Si-Hai2, YANG Yang2, ZHAO Zong-Sheng3, WANG Ya-Chun1, ZHANG Yi1,*
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2 Shihezi Animal Husbandry and Veterinary Workstation, The Eighth Division of Xinjiang Production and Construction Corps, Shihezi 832000, China; 3 College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
Abstract：Simmental cattle (Bos taurus) and its related breeds are the main dual-purpose breeds and beef breeds in China. In recent years, multiple genetic defects, causing early embryonic death, newborn calf defects, reduced fertility and other problems and resulting in economic losses to farms, have been identified in Simmental cattle populations using genomic technologies. This study aimed to develop an accurate and efficient method based on kompetitive allele-specific PCR (KASP) assays to screen 9 genetic defects in Simmental cattle, including Dwarfism, Arachnomelia syndrome, Syndactyly, Thrombopathy, Zinc deficiency-like disorders, Bovine male subfertility, Brown Swiss haplotype 2, Fleckvieh haplotype 2 and Fleckvieh haplotype 4. The call rate was 100% for the 96 tested samples, and carriers were detected on 7 genetic defects. This study provides a technical tool for screening and management of harmful genes during the importation of genetic materials, selection of sires and mating programs of cows in China.
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