日粮中β-胡萝卜素水平对牦牛肠道<em>BCMO1</em>和<em>BCDO2</em>基因及相关代谢调控因子表达的影响及相关性分析

doi:10.3969/j.issn.1674-7968.2021.06.011

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摘要具有黄色体脂的牦牛(Bos grunniens)表现出对类胡萝卜素明显的富集能力。β-胡萝卜素(β-carotene, β-C)为类胡萝卜素的一种,在牦牛类胡萝卜素代谢过程中发挥重要作用。本研究选取15头2~3岁牦牛为研究对象,随机分为5组：对照组饲喂基础日粮;实验组包括低、中、高剂量组,在基础日粮中添加β-C的量分别为720、1 440、1 620 mg/d;阳性对照添加β-C的量为3 440 mg/d。饲养90 d后,利用qRT-PCR分析肠道组织类胡萝卜素相关调控基因—β-胡萝卜素-15,15'-单加氧酶(β-carotene-15,15'-monooxygenase, BCMO1)和β-胡萝卜素-9',10'-双加氧脱氢酶(β-carotene-9',10'-dioxygenase, BCDO2)以及4个相关代谢调控因子基因—肠道特异同源框(intestine specific homeobox, ISX)、过氧化物酶体增殖物激活受体γ (peroxisome proliferator-activated receptor γ, PPARγ)、B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ, SR-B1)及视黄酸受体(retionic acid receptor, RAR)的表达差异;采用Pearson法对上述基因间的表达进行相关性分析。结果显示,在十二指肠中,中剂量组BCMO1表达量显著高于其余剂量组,低、中、高添加量对BCDO2表达无显著影响,低剂量β-C添加更有利于ISX表达,BCDO2与PPARγ、SR-B1间存在显著正相关。在空肠中,低、中剂量组BCMO1表达显著高于其余组,除阳性对照组外BCDO2表达基本不受添加量的影响,BCDO2与ISX、PPARγ、RAR之间存在显著正相关。在回肠中,BCMO1和BCDO2表达变化趋势较平缓,中剂量组有利于BCMO1和BCDO2表达;4个代谢调控因子的表达变化趋势不一,低剂量有利于ISX表达,中剂量有利于SR-B1表达;BCMO1与PPARγ、SR-B1之间存在显著正相关。在盲肠中,BCMO1和BCDO2表达基本不受添加量影响。在结肠中,随添加量的增加BCMO1表达下降;除阳性对照组外,BCDO2表达量不受添加量影响;ISX和RAR表达量都在低剂量组呈现最大值。本研究揭示了日粮β-C添加对牦牛肠道BCMO1和BCDO2及相关代谢调控因子基因表达的影响,为反刍动物体内类胡萝卜素代谢机制研究、牦牛的科学补饲以及优质牦牛肉生产提供基础资料。

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关键词 ：牦牛, β-胡萝卜素, β-胡萝卜素-15,15'-单加氧酶(BCMO1), β-胡萝卜素-9',10'-双加氧脱氢酶(BCDO2), 类胡萝卜素相关代谢调控因子

Abstract：The yak (Bos grunniens) with yellow body fat showed obvious ability to enrich carotenoids. β- carotene (β-C) is a kind of carotenoids, which plays an important role in carotenoid metabolism of yak. In this study, 15 yaks aged 2~3 years were randomly divided into 5 groups: The control group was fed basic diet; The experimental group included low, medium and high-dose groups, in which the amount of added β-C was 720, 1 440 and 1 620 mg/d, respectively; Positive control was added β-C with 3 440 mg/d. After β-C feeding for 90 d, the expression differences of carotenoid related regulatory gene β-carotene-15,15'-momoxygenase (BCMO1), β-carotene-9', 10'-dioxygenase (BCDO2) and 4 related metabolic regulatory factors - intestine specific homeobox (ISX), peroxisome proliferator-activated receptor γ (PPARγ), scavenger receptor class B type Ⅰ (SR-B1) and retionic acid receptor (RAR) were measured by qRT-PCR, and correlation between BCMO1, BCDO2 and related metabolic regulators was analyzed by Pearson method. The results showed that, in yak duodenum, the expression of BCMO1 in middle dose group was significantly higher than that of other groups; Low, medium and high dosage had no significant effect on BCDO2 expression; Low dosage of β-C was more favorable for ISX expression; There was significant positive correlation between BCDO2 and PPARγ, SR-B1. In the jejunum, the expression of BCMO1 in low and middle dose groups was significantly higher than that of other groups; BCDO2 expression was not affected with β-C supplementation except for the positive group; There was significant positive correlation between BCDO2 and ISX, PPARγ, RAR. In the ileum, the expression change of BCMO1 and BCDO2 were smooth, and the medium dose group was beneficial to the expression of BCMO1 and BCDO2; The expression trends of the 4 metabolic regulatory factors were different, and low dose was beneficial to the expression of ISX, and medium dose was beneficial to the expression of SR-B1; There was significant positive correlation between BCMO1 and PPARγ, SR-B1. In the cecum, the expression of BCMO1 and BCDO2 was not affected by the supplementation. In the colon, the expression of BCMO1 increased with the supplementation; Except for the positive group, the BCDO2 expression was not affected by β-C supplementation; The expression of ISX and RAR showed the maximum in low dose group. The present study provides basic materials for mechanism study of carotenoid metabolism in ruminants, scientific supplementary feeding of yak and the production of high quality yak meat.

Key words： Yak &#x003b2 -carotene &#x003b2 -carotene-15 15'-monooxygenase (BCMO1) &#x003b2 -carotene-9' 10'-dioxygenase (BCDO2) Carotenoid related metabolic regulators

收稿日期: 2020-09-22

ZTFLH:

S823.8+5

基金资助:国家自然科学基金(31660665)

通讯作者: * wangsl1970@163.com

引用本文:

任晓莹, 王树林. 日粮中β-胡萝卜素水平对牦牛肠道BCMO1和BCDO2基因及相关代谢调控因子表达的影响及相关性分析[J]. 农业生物技术学报, 2021, 29(6): 1132-1141.
REN Xiao-Ying, WANG Shu-Lin. Effects of Dietary β-carotene Levels on Gene Expression of BCMO1, BCDO2 and Related Metabolic Regulators in Yak (Bos grunniens) Intestine and Correlation Analysis. 农业生物技术学报, 2021, 29(6): 1132-1141.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.06.011 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I6/1132

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