Expression and Localization of NGB and HIF-1α in the Tissues Related to Diencephalon of Yak (Bos grunniens)
MI Xiao-Yu1, DU Xiao-Hua2, DONG Jian-Ying2, James Blackar MAWOLO1, LI Qiao1, WEN Yong-Qiang1, LIU Xia1,*, MA Rui1
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
Abstract：The vertebrate central nervous system is highly sensitive to hypoxia. As a species with long-term adaptability to the hypoxic environment, yak's (Bos grunniens) central nervous system, especially the brain, must have its own special adaptation mechanism. Therefore, it is necessary to study how the yak brain adapts to this hypoxic environment. Several hypoxic neuroprotective factors have been discovered, of which neuroglobin (NGB) and hypoxia-inducible factor-1α (HIF-1α) are mainly expressed in the vertebrate nervous system. But currently, the expression and distribution characteristics of NGB and HIF-1α in yak brain, especially in diencephalon, still remains unclear. This study was aimed to discover the expression and distribution differences or similarities of NGB and HIF-1α in yak's diencephalon and explore the reasons,and qRT-PCR, Western blot and immunohistochemical techniques were used to detect the expression and localizations of NGB and HIF-1α. The results showed that the expression levels of NGB and HIF-1α mRNA were the highest in the pituitary and pineal of yak diencephalon (P<0.05). The expression characteristics of NGB in yak diencephalon were coincident with HIF-1α, and The order from high to low was pituitary, pineal, thalamus, epithalamus, optic nerve and hypothalamus. There was no difference in the expression of NGB and HIF-1α among thalamus, epithalamus, optic nerve and hypothalamus. The expression levels of NGB protein had the highest expression in the pituitary and hypothalamus, while HIF-1α protein had the highest expression in the pituitary (P<0.05). There was no difference in the expression of NGB protein among thalamus, pineal, epithalamus, and optic nerve. There were significant differences in the expression of HIF-1α protein in the yak pituitary, thalamus, epithalamus, and optic nerve. The expression characteristics of NGB in yak diencephalon, including pituitary, thalamus, pineal, epithalamus, optic nerve, were coincident with HIF-1α while the expression characteristics of NGB and HIF-1α was opposite in the hypothalamus. The positive products of NGB and HIF-1α were mainly distributed in the cytoplasm of neurons, and the distribution characteristics of HIF-1α in yak diencephalon were coincident with NGB. The intensity of the immunopositive response of HIF-1α protein was higher than that of NGB protein. NGB and HIF-1α proteins were mainly expressed in the cytoplasm of polymorphic cells of the thalamus, epithalamus, hypothalamus; There were mainly expressed in the cytoplasm of acidophages in the pituitary; in the pineal gland were mainly expressed in the cytoplasm of the pineal cells; There were mainly expressed in the cytoplasm of visual nerve cells and nerve glial cells in the optic nerve, and the 2 proteins were not found in all negative controls. The above results suggested that the pituitary in the yak's diencephalon may have a strong susceptibility to hypoxia. There may be a synergy between NGB and HIF-1α in improving the ability of the yak's diencephalon to adapt the hypoxia environment, however, some regions may have certain regional selectivity depending on their different functions. The coordination and selection mechanisms remain to be determined in further research. This study provides a valuable theoretical basis for further research on the mechanism of yak brain tissues adaptation to hypoxia, and also provides reference data support for in-depth exploration of the possible physiological functions of mammalian brain tissue NGB and HIF-1α.
米晓钰, 杜晓华, 董建英, James Blackar MAWOLO, 李乔, 温永强, 刘霞, 马睿. NGB和HIF-1α在牦牛间脑相关组织中的表达与定位[J]. 农业生物技术学报, 2020, 28(10): 1810-1819.
MI Xiao-Yu, DU Xiao-Hua, DONG Jian-Ying, James Blackar MAWOLO, LI Qiao, WEN Yong-Qiang, LIU Xia, MA Rui. Expression and Localization of NGB and HIF-1α in the Tissues Related to Diencephalon of Yak (Bos grunniens). 农业生物技术学报, 2020, 28(10): 1810-1819.
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