Identification of a Tobacco (Nicotiana tobacum) PGPR Strain and Optimization for Its Fermentation Conditions by Response Surface Analysis Method
WU Xiang*, XIE Li-Yuan, TAN Hao, GAN Bing-Cheng, PENG Wei-Hong, HUANG Zhong-Qian
Soil and Fertilizer institute, Sichuan Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization, Ministry of Agriculture/Key Laboratory of Agricultural Environment in Southwest Mountain Areas, Ministry of Agriculture, Chengdu 610066, China
Abstract：The definite taxonomic classification and fermentation conditions of the functional bacterial could be more efficient for biofertilizer development. Plant growth-promoting rhizobacteria (PGPR) strain MT-002-B-12 is promising isolate for tobacco biofertilizer production as a potential supplementary microbe. The aim of this study is to identify its taxonomic classification, and further optimize the conditions in fermentation. The phylogeny of PGPR strain MT-002-B-12 was analyzed by phenotypic features, physiological-biochemical characteristics and 16S rDNA gene sequence alignments. The fermentation conditions of MT-002-B-12 to produce indole-3-acetic acid (IAA) were optimized by combining the single factor condition analysis and response surface analysis method. The result of classification showed that MT-002-B-12 was Klebsiella variicola. The fermentation experiments showed that the optimum fermentation conditions for MT-002-B-12 was culture volume 30% (V/V), initial pH 6.0, temperature 25 ℃, shaker speed 150 r/min, culture time 34 h; and the optimum content of nutrients in the medium was 1.11% sucrose, 1.36% peptone, 0.15‰ MgSO4·7H2O, 0.34‰ CaCl2·2H2O. The strain MT-002-B-12 enriched the species of growth promoting bacteria, and could be used as a strain resource for the development of tobacco biofertilizer.
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