Abstract：It has great significance to isolate and screen antagonistic endophytic bacteria from the wild Polygonatum cyrtonema and study the antibacterial activity of its metabolites.The endophytic bacteria HJ-2 isolated and screened from the root of the wild P. cyrtonema was identified as Bacillus spp.. In order to furtherly utilize the strain, different mediums were used for fermentation of the strain. The active substances were extracted from the thalli of strain HJ-2 by methanol, ethanol, n-butanol, acetone and ethyl acetate, respectively. Meanwhile, the fermented metabolites were extracted from the fermentation broth by ethyl acetate. All the extracted products were used for physicochemical properties and antibacterial activity study. Our results showed that the antibacterial active substances produced by the strain were present in the fermentation broth and had significant antibacterial effects against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The fermentation products were further tested for acid and alkali resistance and heat resistance. The results showed that the products were material of thermostability (65?C), but had a poor performance of acid and alkali resistance. These results provided data support for the screening of novel antibacterial compounds and the development of this strain.
[1]柏晓辉, 刘孝莲, 刘娣, 等.一株黄精内生菌的分离鉴定及抑菌活性研究[J].天然产物研究与开发, 2018, 30(5):777-782
[2]陈涛风, 王开梅, 万中义, 等.2008. 拮抗放线菌WS-03030的分类鉴定及其抗菌成分的初步研究[J]. 湖北农业科学, 47(4): 427-429. (Chen T F, Wang K M, Wan Z Y, et al. 2008. Classification of Actinomycetes WS-03030 and Its Antifungal Substances[J]. Hubei Agricultural Sciences, 47(4): 427-429.)
[3]黄敬瑜, 张楚军, 姚瑜龙, 等.2017. 植物内生菌生物抗菌活性物质研究进展[J]. 生物工程学报, 33(2): 178-186. (Huang J Y, Zhang C J, Yao Y L, et al. 2017. Progress in antimicrobial substances of endophytes [J]. Chinese Journal of Biotechnology, 33(2): 178-186.)
[4]李艳玲, 王德才, 史仁玖, 等.2013. 泰山黄精内生真菌的分离鉴定及抑菌活性研究[J]. 中草药, 44(11): 1490-1494. (Li Y L, Wang D C, Shi R J, et al. 2013. Isolation and identification of endophytic fungi from Polygonatum sibiricum in Mountain Tai and study on their antimicrobial activity[J]. Chinese Traditional and Herbal Drugs, 44(11): 1490-1494.)
[5]汪玥, 秦婷婷, 纵帅, 等.2016. 2013—2015年金黄色葡萄球菌的临床分布及耐药性分析[J]. 临床与病理杂志, 36(12): 1996-2001. (Wang Y, Qin T T, Zong S, et al. 2016. Clinical distribution and drug resistance of Staphylococcus aureus from 2013 to 2015[J]. Journal of Clinical and Pathological Research, 36(12): 1996-2001.)
[6]汪滢, 王国平, 王丽薇, 等.2010. 一株多花黄精内生真菌的鉴别及其抗菌代谢产物[J]. 微生物学报, 50(8): 1036-1043．(Wang Y, Wang G P, Wang L W, et al. 2010. Isolation and identification of an endophytic fungus of Polygonatum cyrtonema and its antifungal metabolites[J]. ACTA Microbiologica Sinica, 50(8): 1036-1043.)
[7]肖顺, 张婷婷, 卢云彬, 等.藤黄轮丝链霉菌-抑菌活性研究[J].农业生物技术学报, : -., , , . . - []., 2014, 22(11):1226-1329
[8]张峰, 高群, 孔令雷, 等.2007. 黄精多糖抗肿瘤作用的实验研究[J]. 中国实用医药, 2(21): 95-96. (Zhang F, Gao Q, Kong L L, et al. 2007. Anti-tumor effect of polysaccharides isolated from Polygonatum cyrtonema[J]. China Practical Medical, 2(21): 95-96.)
[9]张应烙.2008. 昆虫肠道真菌的新活性物质研究[D]. 博士学位论文, 南京大学, 导师: 谭仁祥, pp. 63-64. (Zhang Y L. 2008. Investigation on novel bioactive metabolites procuced by fungi residing in insect gut[D]. Thesis for Ph.D., Nanjing University, Suppervisor: Tan R X, pp. 63-64.)
[10]朱红艳, 许金俊.1999. 黄精延缓衰老研究进展[J]. 中草药, 30(10): 795-797. (Zhu H Y, Xu J J. 1999. Research progress on anti-aging of Polygonatum cyrtonema[J]. Chinese Traditional and Herbal Drugs, 30(10): 795-797.)
[11]Li W Y, Tailhadesneil J, O’Brien-simpsonfrances N.M., et al. 2014. Proline-rich antimicrobial peptides: potential therapeutics against antibiotic-resistant bacteria[J]. Amino Acids, 46(10): 2287-2294.
