Abstract：Several novel gene editing techniques have been applied in scientific research and livestock breeding in recent years, including zinc finger protein nuclease (ZFN), transcriptional activator-like effector nuclease (TALEN), clustered regularly interspaced short palindromic repeats/CRISPR associated proteins (CRISPR/Cas) system. These gene editing techniques site-directly edit genomes by recognizing the target sites according to the specific structure followed by endonuclease dissection,which is characterized by high efficiency, accuracy, and simple production. In this paper, we demonstrated the basic principles, differences and potential main problems of above three gene editing techniques, summarized the application in the main livestock pigs, cattle and sheep, and provided an outlook of the future of these techniques in application.
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