Abstract：Since the birth of the gene editing technology CRISPR/Cas9, it has been widely used in the fields of gene knockout, knock-in, and base repair. But its inefficiency and off-target cutting, low security, greatly limits the traditional CRISPR/Cas9 application in high-resolution single-base editing. The emergence of the base editor (BE) based on traditional CRISPR/Cas9 overcomes these drawbacks. The cytosine base editor (CBE) converts C•G to T•A, adenine base editor (ABE) enables the conversion of A•T to G•C, enabling efficient single base substitutions without introducing double-strand breaks, which avoids an uncontrollable insertion or deletion mutation (Indels) induced by the traditional CRISPR/Cas9 non-homologous end joining (NHEJ). Most of the genetic diseases in human are caused by base mutations, and the emergence of single-base editing tools can correct a certain proportion of pathogenic SNPs to some extent. Therefore it has broad application prospects in animal model construction, functional genomics research, molecular breeding, clinical medicine, and translational medicine. In this paper, the principle, development, application, opportunities and challenges of the just emerging ABEs with lower off-target efficiency are reviewed, in order to provide reference for the research and application of single-base editing technology.
李广栋, 张鲁, 富俊才, 连正兴, 刘国世. 单碱基编辑工具—腺嘌呤碱基编辑器ABE的研究进展[J]. 农业生物技术学报, 2019, 27(10): 1831-1839.
LI Guang-Dong, ZHANG Lu, FU Jun-Cai, LIAN Zheng-Xing, LIU Guo-Shi. Advances in Research on Single Base Editing Tool--Adenine Base Editor ABE. 农业生物技术学报, 2019, 27(10): 1831-1839.
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