Mining and Verification of SNP Loci of Ustilago esculenta Used for Grey Jiaobai Identification
WU Jun-Cheng1,*, GE Xin-Tao2,*, YANG Meng-Fei3, YE Zi-Hong1, XIA Wen-Qiang1, TANG Jin-Tian1, YU Xiao-Ping1, ZHANG Shang-Fa3, ZHANG Ya-Fen1,**
1 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China; 2 Hangzhou C&Y Pollen Pharmaceutical Co., Ltd., Hangzhou 310018, China; 3 Jinghua City Academy of Agricultural Sciences, Jinghua 321017, China
Abstract：Jiaobai as an important aquatic vegetable in China, nearly 20% of the grey Jiaobai in the field has a serious impact on its yield. Previous research showed that the formation of 2 phenotypes of normal Jiaobai and grey Jiaobai in the field was closely related to the differentiation of MT and T type strains of Ustilago esculenta. Grey Jiaobai is caused by the infection of Zizania latifolia by T type strain of U. esculenta. Therefore, 129 494 SNP loci with difference between MT and T-type strains was obtained in this study, by resequencing, SNP calling and SNP filtration. After that, 647 specific SNP loci with specificity of T type of strain were screened by Hardy-Weinberg equilibrium test strategy and short fragment sieving. Finally, based on the 48 loci selected by randomization principle, 8 loci with high accuracy were screened by allele specific PCR (AS-PCR) with designed specific primers for field test. The results showed that the occurrence ratio of grey Jiaobai could be reduced 37.59% after eliminating the seedlings with positive identification results using the combination of the selected 8 specific loci to identify the T-type strain. This research provides technical support for further improving the purity of seedlings in improved seed breeding and subsequent seedling quality detection.
吴骏成, 葛鑫涛, 杨梦飞, 叶子弘, 夏文强, 汤近天, 俞晓平, 张尚法, 张雅芬. 用于灰茭鉴别的菰黑粉菌SNP位点挖掘及验证[J]. 农业生物技术学报, 2023, 31(2): 383-392.
WU Jun-Cheng, GE Xin-Tao, YANG Meng-Fei, YE Zi-Hong, XIA Wen-Qiang, TANG Jin-Tian, YU Xiao-Ping, ZHANG Shang-Fa, ZHANG Ya-Fen. Mining and Verification of SNP Loci of Ustilago esculenta Used for Grey Jiaobai Identification. 农业生物技术学报, 2023, 31(2): 383-392.
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