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2025年4月5日 星期六
农业生物技术学报  2022, Vol. 30 Issue (1): 85-95    DOI: 10.3969/j.issn.1674-7968.2022.01.008
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
基于RNA-seq技术挖掘影响贵州黄鸡肌内脂肪沉积的关键基因
王小亚1,2,3,4, 陈大海1,2,3,4, 饶永超1,2,3, 杨德凤1,2,3, 唐宏1,2,3, 林家栋1,2,3,4, 张福平1,2,3,4,*
1贵州大学 高原山地动物遗传育种与繁殖教育部重点实验室,贵阳 550025;
2贵州省动物遗传育种与繁殖重点实验室;贵阳 550025;
3贵州大学 动物科学学院,贵阳 550025;
4贵州大学 科研鸡场,贵阳 550025
Exploration of Key Genes Affecting Intramuscular Fat Deposition in Guizhou Yellow Chicken (Gallus gallus domesticus) Based on RNA-seq Technology
WANG Xiao-Ya1,2,3,4, CHEN Da-Hai1,2,3,4, RAO Yong-Chao1,2,3, YANG De-Feng1,2,3, TANG Hong1,2,3, LIN Jia-Dong1,2,3,4, ZHANG Fu-Ping1,2,3,4,*
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
2 Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guiyang 550025, China;
3 College of Animal Science, Guizhou University, Guiyang 550025, China;
4 Research Chicken Farm of Guizhou University Guiyang 550025, China
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摘要 肌内脂肪(intramuscular fat, IMF)含量是衡量肉品质的重要指标之一。但肌内脂肪含量的沉积受遗传、环境和营养水平因素的影响。不同的品种、品系,甚至同一品系不同个体间肌内脂肪的沉积均有差异。本研究以贵州黄鸡(Gallus gallus domesticus)为研究对象,旨在探讨不同个体间IMF含量的差异,并通过转录组测序(RNA-seq)技术分析贵州黄鸡鸡肉中IMF沉积的分子调控机理。选择相同条件下饲养、体重相近的120日龄贵州黄鸡母鸡30只,屠宰后逐只测定胸肌IMF含量。根据肌内脂肪含量高低分为2组:高IMF组(H组, n=4)和低IMF组(L组, n=4)。采用RNA-seq技术测序,挖掘影响贵州黄鸡肌内脂肪沉积的差异表达基因,并对差异表达基因进行GO功能注释和KEGG通路富集分析。结果发现,高IMF含量和低IMF含量2个组胸肌组织测序共筛选出259个差异表达基因,其中有64个基因表达上调,195个基因表达下调;随机选择6个基因进行qPCR验证,发现qPCR结果与测序结果一致,证明测序结果可靠。KEGG信号通路分析发现粘着斑、细胞外基质(extracellular matrix, ECM)-受体相互作用、肌动蛋白细胞骨架调节、细胞黏附分子和TGF (transforming growth factor)-β信号通路等5条信号通路显著富集。在粘着斑、ECM-受体相互作用、TGF-β信号通路与PPAR信号通路上发现钙蛋白酶2 (calproteinase 2, CAPN2)、-1型胶原蛋白链(collagen type alpha 1 chain, COL1A1)、COL1A2、胶原型α1链(collagen type alpha 1 chain, COL6A1)、COL6A2COL6A3和脂蛋白1 (lipin1, LPIN1)、磷脂转运蛋白(phospholipid transfer protein, PLTP)等基因是影响贵州黄鸡IMF沉积的关键基因。本研究所鉴定差异表达基因可作为IMF沉积的关键基因,为今后进一步探索家禽IMF沉积的分子调控机理提供参考。
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王小亚
陈大海
饶永超
杨德凤
唐宏
林家栋
张福平
关键词 贵州黄鸡转录组测序(RNA-seq)技术肌内脂肪沉积候选基因    
Abstract:Intramuscular fat (IMF) content was one of the important indicators to measure meat quality. However, the deposition of intramuscular fat content was affected by heredity, environment and nutrition level, and the deposition of intramuscular fat content was different among different varieties and strains, even among different individuals of the same strain. The aim of this study was to explore the differences in IMF content among different individuals of Guizhou yellow chickens (Gallus gallus domesticus) and to analyze the molecular regulation mechanism of IMF deposition in muscle of Guizhou yellow chickens by RNA-seq technique. 30 Guizhou yellow chickens hens were selected and raised in the same conditions to 120 d of age, and the IMF content in breast muscle was determined one by one after slaughter. There were 2 groups according to the level of IMF content: The high-IMF group (H group, n=4) and the low-IMF group (L group, n=4). In this study, RNA-seq technique was used to screen differentially expressed genes affecting IMF deposition in breast muscle of Guizhou yellow chicken; It also analyzed the differentially expressed genes for GO functional annotation and KEGG pathway enrichment. The results showed that a total of 259 differentially expressed genes were screened out from breast muscle tissues of individuals with high and low IMF content, including 64 up-regulated genes and 195 down-regulated genes. 6 genes were randomly selected for qPCR validation, and the qPCR results were consistent with the sequencing results, proved that the sequencing results were reliable. KEGG signaling pathway analysis showed that 5 signaling pathways, including focal adhesion, extracellular matrix (ECM) -receptor interaction, regulation of actin cytoskeleton, cell adhesion molecules, and TGF (transforming growth factor)-β signaling pathway, these pathways were significantly enriched. Calproteinase 2 (CAPN2), collagen type alpha 1 chain (COL1A1), COL1A2, collagen type alpha 1 chain (COL6A1), COL6A2, COL6A3, lipin1 (LPIN1) and phospholipid transfer protein (PLTP) were found in adhesion spots, ECM-receptor interaction, TGF-beta signaling pathway and PPAR signaling pathway. These genes may be key genes affecting IMF deposition in Guizhou yellow chickens. The differential expression genes identified in this study can be used as key genes for IMF deposition and provide a reference to explore the molecular regulation mechanism of IMF deposition in poultry in future.
Key wordsGuizhou yellow chicken    RNA-seq    Intramuscular fat deposition    Candidate gene
收稿日期: 2021-07-01     
ZTFLH:  S831  
基金资助:贵州省科技计划项目(黔科合支撑[2019]2288号)
通讯作者: *zfu-1010@126.com   
引用本文:   
王小亚, 陈大海, 饶永超, 杨德凤, 唐宏, 林家栋, 张福平. 基于RNA-seq技术挖掘影响贵州黄鸡肌内脂肪沉积的关键基因[J]. 农业生物技术学报, 2022, 30(1): 85-95.
WANG Xiao-Ya, CHEN Da-Hai, RAO Yong-Chao, YANG De-Feng, TANG Hong, LIN Jia-Dong, ZHANG Fu-Ping. Exploration of Key Genes Affecting Intramuscular Fat Deposition in Guizhou Yellow Chicken (Gallus gallus domesticus) Based on RNA-seq Technology. 农业生物技术学报, 2022, 30(1): 85-95.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.01.008     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I1/85
 
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