Preliminary Development of a Test Strip for Rapid Detection of Antibodies Against African swine fever virus
JIANG Di-Ke1,*, WU Xu-Long2,*, CHEN Di-Shi3, WANG Yin1,**, YAO Xue-Ping1, LUO Yan1, YANG Ze-Xiao1, ZHANG Peng-Fei1
1 College of Veterinary Medicine/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
2 Chengdu Agricultural College, Chengdu 611130, China;
3 Sichuan Center for Animal Disease Control and Prevention, Chengdu 610041, China
Abstract：In order to establish a rapid, efficient and simple method for detecting African swine fever virus (ASFV) antibodies, this study used a prokaryotic expression system to express ASFV capsid protein (p72) as a capture antigen, and anti-p72 polyclonal antibody and rabbit anti-pig IgG were coated on nitrocellulose membrane, as the quality control line (C line) and detection line (T line), respectively. The reaction conditions were optimized to prepare colloidal gold immunochromatographic test strip (ICTS), used for clinical sample detection. The results showed that the pCold-TF-p72 recombinant protein was successfully constructed in this study. Western blot and agar expansion experiments showed that the prepared anti-p72 polyclonal antibody had good reactogenicity and immunogenicity. The test strips could specifically detect anti-ASFV antibody and had no cross-reaction with positive sera against Pseudorabies virus, Porcine circovirus 2, Porcine reproductive and respiratory syndrome virus and Classical swine fever virus; In the detection of clinical samples, the positive detection rate of ICTS was 55.17% (48/87), the positive detection rate of a commercial ELISA kit was 59.77% (52/87), respectively. The agreement between ICTS and a commercial ELISA kit (IDVET) was 92.31% (48/52). The ICTS prepared in this study provides a reference for clinical ASFV antibody detection and disease prevention.
江地科, 邬旭龙, 陈弟诗, 王印, 姚学萍, 罗燕, 杨泽晓, 张鹏飞. 非洲猪瘟病毒抗体快速检测试纸条的初步研制[J]. 农业生物技术学报, 2022, 30(1): 185-193.
JIANG Di-Ke, WU Xu-Long, CHEN Di-Shi, WANG Yin, YAO Xue-Ping, LUO Yan, YANG Ze-Xiao, ZHANG Peng-Fei. Preliminary Development of a Test Strip for Rapid Detection of Antibodies Against African swine fever virus. 农业生物技术学报, 2022, 30(1): 185-193.
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